Nuclear phosphoinositides are recognized as regulators of many nuclear processes including chromatin remodeling, splicing, transcription, DNA repair and epigenetics. These processes are spatially organized in different nuclear compartments. Phase separation is involved in the formation of various nuclear compartments and molecular condensates separated from surrounding environment. The surface of such structures spatiotemporally coordinates formation of protein complexes. PI(4,5)P2 (PIP2) integration into phase-separated structures might provide an additional step in their spatial diversification by attracting certain proteins with affinity to PIP2. Our laboratory has recently identified novel membrane-free PIP2-containing structures, so called Nuclear Lipid Islets (NLIs). We provide an evidence that these structures are evolutionary conserved in different organisms. We hypothesize that NLIs serve as a scaffolding platform which facilitates the formation of transcription factories, thus participating in the formation of nuclear architecture competent for transcription. In this review we speculate on a possible role of NLIs in the integration of various processes linked to RNAPII transcription, chromatin remodeling, actin-myosin interaction, alternative splicing and lamin structures.

Department of Biology of the Cell Nucleus Institute of Molecular Genetics of the Czech Academy of Sciences v v i 142 20 Prague Czech Republic

Department of Biology of the Cell Nucleus Institute of Molecular Genetics of the Czech Academy of Sciences v v i 142 20 Prague Czech Republic; Department of Epigenetics of the Cell Nucleus Institute of Molecular Genetics of the Czech Academy of Sciences v v i division BIOCEV 25250 Vestec Czech Republic; Microscopy Centre Institute of Molecular Genetics of the Czech Academy of Sciences v v i 142 20 Prague Czech Republic

Citace poskytuje Crossref.org

Plasma membrane and nuclear phosphatidylinositol 4,5-bisphosphate signalling in cancer

The RNA-dependent association of phosphatidylinositol 4,5-bisphosphate with intrinsically disordered proteins contribute to nuclear compartmentalization

Lamin A/C and PI(4,5)P2-A Novel Complex in the Cell Nucleus

Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts

How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription

The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus

Limited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome

Tidying-up the plant nuclear space: domains, functions, and dynamics

Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome

Nuclear Phosphoinositides-Versatile Regulators of Genome Functions