Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- AFM, TEM, THP-1 cells, cathepsin B, cathodoluminescence, cell immunopathology, fluorescent nanodiamonds, inflammasome NLRP3, inflammation, lysosomal disruption, macropinocytosis, nanodiamond intercellular distribution,
- MeSH
- Cell Membrane drug effects metabolism ultrastructure MeSH
- Dynamic Light Scattering MeSH
- Microscopy, Electron MeSH
- Fluorescence MeSH
- Inflammasomes drug effects immunology metabolism MeSH
- Intravital Microscopy methods MeSH
- Cathepsin B immunology metabolism MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Lysosomes drug effects immunology metabolism ultrastructure MeSH
- Microscopy, Atomic Force MeSH
- Nanodiamonds administration & dosage chemistry MeSH
- Pinocytosis MeSH
- NLR Family, Pyrin Domain-Containing 3 Protein immunology metabolism MeSH
- THP-1 Cells MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CTSB protein, human MeSH Browser
- Inflammasomes MeSH
- Cathepsin B MeSH
- Nanodiamonds MeSH
- NLRP3 protein, human MeSH Browser
- NLR Family, Pyrin Domain-Containing 3 Protein MeSH
Nanodiamonds (ND), especially fluorescent NDs, represent potentially applicable drug and probe carriers for in vitro/in vivo applications. The main purpose of this study was to relate physical-chemical properties of carboxylated NDs to their intracellular distribution and impact on membranes and cell immunity-activation of inflammasome in the in vitro THP-1 cell line model. Dynamic light scattering, nanoparticle tracking analysis, and microscopic methods were used to characterize ND particles and their intracellular distribution. Fluorescent NDs penetrated the cell membranes by both macropinocytosis and mechanical cutting through cell membranes. We proved accumulation of fluorescent NDs in lysosomes. In this case, lysosomes were destabilized and cathepsin B was released into the cytoplasm and triggered pathways leading to activation of inflammasome NLRP3, as detected in THP-1 cells. Activation of inflammasome by NDs represents an important event that could underlie the described toxicological effects in vivo induced by NDs. According to our knowledge, this is the first in vitro study demonstrating direct activation of inflammasome by NDs. These findings are important for understanding the mechanism(s) of action of ND complexes and explain the ambiguity of the existing toxicological data.
Institute of Scientific Instruments Czech Academy of Sciences Brno 61264 Czech Republic
References provided by Crossref.org
Immunotoxicity of Carbon-Based Nanomaterials, Starring Phagocytes
