Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- AFM, TEM, THP-1 cells, cathepsin B, cathodoluminescence, cell immunopathology, fluorescent nanodiamonds, inflammasome NLRP3, inflammation, lysosomal disruption, macropinocytosis, nanodiamond intercellular distribution,
- MeSH
- buněčná membrána účinky léků metabolismus ultrastruktura MeSH
- dynamický rozptyl světla MeSH
- elektronová mikroskopie MeSH
- fluorescence MeSH
- inflamasomy účinky léků imunologie metabolismus MeSH
- intravitální mikroskopie metody MeSH
- kathepsin B imunologie metabolismus MeSH
- konfokální mikroskopie MeSH
- lidé MeSH
- lyzozomy účinky léků imunologie metabolismus ultrastruktura MeSH
- mikroskopie atomárních sil MeSH
- nanodiamanty aplikace a dávkování chemie MeSH
- pinocytóza MeSH
- protein NLRP3 imunologie metabolismus MeSH
- THP-1 buňky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CTSB protein, human MeSH Prohlížeč
- inflamasomy MeSH
- kathepsin B MeSH
- nanodiamanty MeSH
- NLRP3 protein, human MeSH Prohlížeč
- protein NLRP3 MeSH
Nanodiamonds (ND), especially fluorescent NDs, represent potentially applicable drug and probe carriers for in vitro/in vivo applications. The main purpose of this study was to relate physical-chemical properties of carboxylated NDs to their intracellular distribution and impact on membranes and cell immunity-activation of inflammasome in the in vitro THP-1 cell line model. Dynamic light scattering, nanoparticle tracking analysis, and microscopic methods were used to characterize ND particles and their intracellular distribution. Fluorescent NDs penetrated the cell membranes by both macropinocytosis and mechanical cutting through cell membranes. We proved accumulation of fluorescent NDs in lysosomes. In this case, lysosomes were destabilized and cathepsin B was released into the cytoplasm and triggered pathways leading to activation of inflammasome NLRP3, as detected in THP-1 cells. Activation of inflammasome by NDs represents an important event that could underlie the described toxicological effects in vivo induced by NDs. According to our knowledge, this is the first in vitro study demonstrating direct activation of inflammasome by NDs. These findings are important for understanding the mechanism(s) of action of ND complexes and explain the ambiguity of the existing toxicological data.
Institute of Scientific Instruments Czech Academy of Sciences Brno 61264 Czech Republic
Citace poskytuje Crossref.org
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