Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

Bijvoet Center for Biomolecular Research Utrecht University Utrecht the Netherlands

Biogen Idec Cambridge MA USA

Codiak Biosciences Cambridge MA USA

Department of Biochemistry and Biophysics University of Pennsylvania Philadelphia PA USA

Department of Biochemistry and Microbiology University of Victoria Victoria BC Canada

Department of Biochemistry and Molecular Biology University of Calgary Calgary AB Canada

Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej Odense Denmark

Department of Biochemistry University of Colorado Boulder CO USA

Department of Biological Chemistry The Hebrew University of Jerusalem Jerusalem Israel

Department of Biological Science National University of Singapore Singapore Singapore

Department of Chemistry and Chemical Biology Northeastern University Boston MA USA

Department of Chemistry The University of Western Ontario London ON Canada

Department of Chemistry University of California Berkeley Berkeley CA USA

Department of Chemistry University of Massachusetts Amherst Amherst MA USA

Department of Chemistry Washington University in St Louis St Louis MO USA

Department of Chemistry York University Toronto ON Canada

Department of Early Stage Pharmaceutical Development Genentech Inc South San Francisco CA USA

Department of Integrative Structural and Computational Biology Scripps Florida The Scripps Research Institute Jupiter FL USA

Department of Medicinal Chemistry School of Pharmacy University of Washington Seattle WA USA

Department of Molecular Medicine The Scripps Research Institute Jupiter FL USA

Department of Pharmaceutical Chemistry University of Kansas Lawrence KS USA

Department of Pharmaceutical Sciences University of Maryland School of Pharmacy Baltimore MD USA

Department of Pharmacy University of Copenhagen Copenhagen Denmark

Genome BC Proteomics Centre University of Victoria Victoria BC Canada

Institut Pasteur Chemistry and Structural Biology Department Paris France

Institute of Microbiology Czech Academy of Sciences Prague Czech Republic

Johnson and Johnson Pharmaeutical Research and Development Jersey City NJ USA

MRC Laboratory of Molecular Biology Cambridge UK

Novo Nordisk Måløv Denmark

Process Development Amgen Thousand Oaks CA USA

Roche Innovation Center Penzberg Penzberg Germany

Roy J Carver Department of Biochemistry Biophysics and Molecular Biology Iowa State University Ames IA USA

UCB Celltech Slough UK

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