The aim of this work was to investigate the thermophilic bacterium Schelegelella thermodepolymerans DSM 15344 in terms of its polyhydroxyalkanoates (PHA) biosynthesis capacity. The bacterium is capable of converting various sugars into PHA with the optimal growth temperature of 55 °C; therefore, the process of PHA biosynthesis could be robust against contamination. Surprisingly, the highest yield was gained on xylose. Results suggested that S. thermodepolymerans possess unique xylose metabolism since xylose is utilized preferentially with the highest consumption rate as compared to other sugars. In the genome of S. thermodepolymerans DSM 15344, a unique putative xyl operon consisting of genes responsible for xylose utilization and also for its transport was identified, which is a unique feature among PHA producers. The bacterium is capable of biosynthesis of copolymers containing 3-hydroxybutyrate and also 3-hydroxyvalerate subunits. Hence, S.thermodepolymerans seems to be promising candidate for PHA production from xylose rich substrates.

Department of Biomedical Engineering Faculty of Electrical Engineering and Communication Brno University of Technology Technicka 10 616 00 Brno Czech Republic

Department of Food Chemistry and Biotechnology Faculty of Chemistry Brno University of Technology Purkynova 118 612 00 Brno Czech Republic

Department of Physical and Applied Chemistry Faculty of Chemistry Brno University of Technology Purkynova 118 612 00 Brno Czech Republic

Institute of Chemistry NAWI Graz University of Graz Heinrichstrasse 28 6 8010 Graz Austria; ARENA Arbeitsgemeinschaft für Ressourcenschonende and Nachhaltige Technologien Inffeldgasse 21b 11 8010 Graz Austria

References provided by Crossref.org

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First Complete Genome of the Thermophilic Polyhydroxyalkanoates-Producing Bacterium Schlegelella thermodepolymerans DSM 15344