Bark beetles often serve as forest damaging agents, causing landscape-level mortality. Understanding the biology and ecology of beetles are important for both, gathering knowledge about important forest insects and forest protection. Knowledge about the bark beetle gut-associated bacteria is one of the crucial yet surprisingly neglected areas of research with European tree-killing bark beetles. Hence, in this study, we survey the gut bacteriome from five Ips and one non-Ips bark beetles from Scolytinae. Results reveal 69 core bacterial genera among five Ips beetles that may perform conserved functions within the bark beetle holobiont. The most abundant bacterial genera from different bark beetle gut include Erwinia, Sodalis, Serratia, Tyzzerella, Raoultella, Rahnella, Wolbachia, Spiroplasma, Vibrio, and Pseudoxanthomonas. Notable differences in gut-associated bacterial community richness and diversity among the beetle species are observed. Furthermore, the impact of sampling location on the overall bark beetle gut bacterial community assemblage is also documented, which warrants further investigations. Nevertheless, our data expanded the current knowledge about core gut bacterial communities in Ips bark beetles and their putative function such as cellulose degradation, nitrogen fixation, detoxification of defensive plant compounds, and inhibition of pathogens, which could serve as a basis for further metatranscriptomics and metaproteomics investigations.

Department of Plant Protection Biology Swedish University of Agricultural Sciences 230 53 Alnarp Sweden

EVA 4 0 Unit Faculty of Forestry and Wood Sciences Czech University of Life Sciences Prague Kamýcká 129 Suchdol 165 21 Prague 6 Czech Republic

Excellent Team for Mitigation Faculty of Forestry and Wood Sciences Czech University of Life Sciences Prague Kamýcká 129 Suchdol 165 21 Prague 6 Czech Republic

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The gut microbiome mediates adaptation to scarce food in Coleoptera

Genome and transcriptome of Ips nitidus provide insights into high-altitude hypoxia adaptation and symbiosis

Impact of transgenerational host switch on gut bacterial assemblage in generalist pest, Spodoptera littoralis (Lepidoptera: Noctuidae)

New insight into the bark beetle ips typographus bacteriome reveals unexplored diversity potentially beneficial to the host

Impact of Wood Age on Termite Microbial Assemblages

Epigenetic regulations as drivers of insecticide resistance and resilience to climate change in arthropod pests

Molecular Rationale of Insect-Microbes Symbiosis-From Insect Behaviour to Mechanism

Reference Gene Selection for Normalizing Gene Expression in Ips Sexdentatus (Coleoptera: Curculionidae: Scolytinae) Under Different Experimental Conditions

A highly-contiguous genome assembly of the Eurasian spruce bark beetle, Ips typographus, provides insight into a major forest pest

Interactions among Norway spruce, the bark beetle Ips typographus and its fungal symbionts in times of drought

A New Perspective of Pseudomonas-Host Interactions: Distribution and Potential Ecological Functions of the Genus Pseudomonas within the Bark Beetle Holobiont