Satellite DNA (satDNA) is a rapidly evolving class of tandem repeats, with some monomers being involved in centromere organization and function. To identify repeats associated with (peri)centromeric regions, we investigated satDNA across Southern and Coastal clades of African annual killifishes of the genus Nothobranchius. Molecular cytogenetic and bioinformatic analyses revealed that two previously identified satellites, designated here as NkadSat01-77 and NfurSat01-348, are associated with (peri)centromeres only in one lineage of the Southern clade. NfurSat01-348 was, however, additionally detected outside centromeres in three members of the Coastal clade. We also identified a novel satDNA, NrubSat01-48, associated with (peri)centromeres in N. foerschi, N. guentheri, and N. rubripinnis. Our findings revealed fast turnover of satDNA associated with (peri)centromeres and different trends in their evolution in two clades of the genus Nothobranchius.

Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic

Department of Ecology and Vertebrate Zoology University of Łódź Łódź Poland

Department of Ecology Faculty of Science Charles University Prague Czech Republic

Department of Experimental Biology Genetics Area University of Jaén Jaén Spain

Department of Genetics and Microbiology Faculty of Science Charles University Prague Czech Republic

Department of Zoology Faculty of Science Charles University Prague Czech Republic

Faculty of Science University of South Bohemia České Budějovice Czech Republic

Institute of Animal Physiology and Genetics Czech Academy of Sciences Liběchov Czech Republic

Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic

Severtsov Institute of Ecology and Evolution Russian Academy of Sciences Moscow Russia

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