The nuclear envelope (NE) separates translation and transcription and is the location of multiple functions, including chromatin organization and nucleocytoplasmic transport. The molecular basis for many of these functions have diverged between eukaryotic lineages. Trypanosoma brucei, a member of the early branching eukaryotic lineage Discoba, highlights many of these, including a distinct lamina and kinetochore composition. Here, we describe a cohort of proteins interacting with both the lamina and NPC, which we term lamina-associated proteins (LAPs). LAPs represent a diverse group of proteins, including two candidate NPC-anchoring pore membrane proteins (POMs) with architecture conserved with S. cerevisiae and H. sapiens, and additional peripheral components of the NPC. While many of the LAPs are Kinetoplastid specific, we also identified broadly conserved proteins, indicating an amalgam of divergence and conservation within the trypanosome NE proteome, highlighting the diversity of nuclear biology across the eukaryotes, increasing our understanding of eukaryotic and NPC evolution.

Biology Centre Czech Academy of Sciences Institute of Parasitology České Budějovice Czech Republic

Department of Pathology University of Cambridge Cambridge UK

Laboratory of Cellular and Structural Biology The Rockefeller University New York NY USA

Laboratory of Mass Spectrometry and Gaseous Ion Chemistry The Rockefeller University New York NY USA

School of Life Sciences University of Dundee Dundee UK

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On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa