The presence of phytase activity was demonstrated in 26 strains of rabbit cecal bacteria. In 25 strains a low phytase activity, 0.10-0.62 micromol phosphate released per min per mg protein, was found. High activity (2.61 micromol/min per mg protein) was found in the strain PP2 identified as Enterococcus hirae. Phytase activity was cell-associated, being higher in the cell extract than in the cell walls. Extracellular phytase activity and cell-associated phosphatase activity were not detected. Phytase activity was optimal around pH 5.0, which is below the physiological cecal pH range. The K (m) determined using the Lineweaver-Burk plot was 0.19 micromol/mL. Cations Fe(3+), Cu(2+) and Zn(2+) at 0.5 mmol/L decreased phytase activity in sonicated cells of E. hirae by 99.4, 90.7 and 96.5 %, respectively. In contrast, Mg(2+) increased activity by 11.0 %. Characteristics of E. hirae phytase (pH optimum, K (m), cation sensitivity) were similar to those of other bacterial phytases reported in the literature. Other bacteria with a high phytase activity may be present in the rabbit cecum but remain to be identified.
- MeSH
- 6-fytasa chemie metabolismus MeSH
- Bacteria enzymologie chemie izolace a purifikace MeSH
- bakteriální proteiny chemie metabolismus MeSH
- cékum mikrobiologie MeSH
- Enterococcus genetika chemie izolace a purifikace MeSH
- financování organizované MeSH
- kinetika MeSH
- králíci MeSH
- stabilita enzymů MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
The novel chitinolytic bacterium Clostridium beijerinckii strain JM2 was isolated from the stool of healthy volunteers supplied daily per orally with 3 g of chitosan. The bacterium grown on colloidal chitin produced a complete array of chitinolytic enzymes. Significant activities of endochitinase, exochitinase and chitosanase were excreted into the medium (301, 282 and 268 nkat/microg protein, respectively). The high cellular activity of N-acetyl-beta-glucosaminidase (NAGase) and chitosanase were detected (732.4 and 154 nkat/microg protein, respectively). NAGase activity represented the main activity associated with the cellular fraction. The activities of both enzymes tested increased from 20 to 50 degrees C; the optimum reaction temperature estimated being 50 degrees C. Endochitinase as well as NAGase showed an activity in the pH interval of 4.0-8.0; the optimum pH values were 6.5 and 6.0, respectively. The extracellular endochitinase complex consisted of six isoenzymes with molar mass of 32-76 kDa; in the cellular fraction five bands with molar mass of 45-86 kDa were detected. Exochitinase activity was demonstrated in the form of three bands (with molar mass of 30-57 kDa), NAGase activity displayed one band of 45 kDa.
- MeSH
- acetylglukosaminidasa metabolismus MeSH
- chitin metabolismus MeSH
- chitinasy metabolismus MeSH
- chitosan aplikace a dávkování MeSH
- Clostridium enzymologie izolace a purifikace klasifikace růst a vývoj MeSH
- feces mikrobiologie MeSH
- glykosidhydrolasy metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- lidé MeSH
- stabilita enzymů MeSH
- teplota MeSH
- Check Tag
- lidé MeSH
- MeSH
- Actinomyces enzymologie izolace a purifikace růst a vývoj MeSH
- amylasy izolace a purifikace MeSH
- Bacteroides enzymologie izolace a purifikace růst a vývoj MeSH
- cékum mikrobiologie MeSH
- Clostridium enzymologie izolace a purifikace růst a vývoj MeSH
- Dichelobacter nodosus enzymologie izolace a purifikace růst a vývoj MeSH
- Eubacterium enzymologie izolace a purifikace růst a vývoj MeSH
- financování vládou MeSH
- králíci mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- králíci mikrobiologie MeSH
- zvířata MeSH
- MeSH
- bachor fyziologie mikrobiologie MeSH
- financování vládou MeSH
- gramnegativní anaerobní bakterie enzymologie růst a vývoj MeSH
- konjugované kyseliny linolové izolace a purifikace MeSH
- mastné kyseliny MeSH
- skot fyziologie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- skot fyziologie mikrobiologie MeSH
- zvířata MeSH
- MeSH
- acetylglukosaminidasa chemie izolace a purifikace MeSH
- chitin chemie MeSH
- chitinasy chemie izolace a purifikace MeSH
- chromatografie iontoměničová metody MeSH
- Clostridium enzymologie izolace a purifikace MeSH
- feces mikrobiologie MeSH
- finanční podpora výzkumu jako téma MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- bachor mikrobiologie MeSH
- finanční podpora výzkumu jako téma MeSH
- fylogeneze MeSH
- geny rRNA genetika MeSH
- houby cytologie genetika izolace a purifikace MeSH
- klasifikace metody MeSH
- sekvenční analýza DNA statistika a číselné údaje využití MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
