Infrared attenuated total reflection spectroscopy was used for in situ observation of the deposition of collagen I on poly(2-hydroxyethyl methacrylate-co-methacrylic acid, 2.9%) hydrogels and subsequent attachment of laminin or fibronectin on the collagen surface. While there was no adsorption of collagen dissolved in an acid solution on the hydrogel surface, it deposited on the surface at pH 6.5. The collagen layers with attached laminin or fibronectin were stable on hydrogel surface in physiological solution. The modification with collagen and particularly with collagen and laminin or fibronectin allowed the adhesion and growth of mesenchymal stromal cells and astrocytes on the hydrogel surface.
- MeSH
- extracelulární matrix - proteiny * chemie MeSH
- hydrogely * chemie MeSH
- koncentrace vodíkových iontů MeSH
- mikroskopie atomárních sil MeSH
- polyhydroxyethylmethakrylát * analogy a deriváty chemie MeSH
- povrchové vlastnosti MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- adsorpce MeSH
- albuminy chemie MeSH
- elektrolyty chemie MeSH
- finanční podpora výzkumu jako téma MeSH
- heparin chemie MeSH
- imunoglobuliny chemie MeSH
- koncentrace vodíkových iontů MeSH
- lidé MeSH
- spektroskopie infračervená s Fourierovou transformací metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH