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Článek

Gene expression profiling after angiogenesis inhibitor treatment

Lékař a technika. 2014 ; 44 (1) : 33-38.

Lék. tech.
ISSN 0301-5491
Medvik
Zdroj

Klíčová slova
photodynamická terapie,
MeSH
cévy fyziologie růst a vývoj sekrece účinky léků MeSH
exprese genu účinky léků MeSH
fibroblastové růstové faktory MeSH
fotochemoterapie využití MeSH
fotosenzibilizující látky MeSH
inhibitory angiogeneze * aplikace a dávkování terapeutické užití MeSH
kyselina salicylová aplikace a dávkování MeSH
látky indukující angiogenezi MeSH
lidé MeSH
nádory * terapie MeSH
prospektivní studie MeSH
TNF-alfa toxicita MeSH
vaskulární endoteliální růstové faktory sekrece MeSH
Check Tag
lidé MeSH
Publikační typ
grafy a diagramy MeSH
práce podpořená grantem MeSH

Článek

Study of photodynamic effects on NIH 3T3 cell line and bacteria

Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic. 2014 ; 158 (2) : 201-207. [pub] 20120615

Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print)
ISSN 1213-8118
Medvik
Zdroj

BACKGROUND: Bacterial resistance to antibiotics is a constantly growing challenge. Photodynamic therapy (PDT) offers a new approach to the treatment of bacterial and viral diseases. The aim of this study was to compare the efficacy of photosensitizers used in PDT applied to cell lines and bacterial strains. METHODS: We tested the cytotoxicity and phototoxicity of 3 photosensitizers: TPPS4, ZnTPPS4 and TMPyP applied to the NIH3T3 cell line using two established methods for measuring ROS production and, MTT viability assay. Bacterial viability was determined spectrophotometrically over 24 h following PDT. RESULTS: The most efficient photosensitiser was TMPyP as it reduced the viability of the NIH3T3 cell line by more than 85%. In general, the photosensitisers were more phototoxic to the two Gram-positive bacterial strains, Enterococcus faecalis and Staphylococcus aureus. The viability of E. faecalis was reduced to 78 % by a dose radiation 0.5 J/cm(2) and concentration of TMPyP 1.562 μmol/L. The viability of bacterium S. aureus was reduced to 23 % when exposed to a radiation dose 0.5 J/cm(2) and 100 μmol/L concentration of ZnTPPS4. The highest viability decrease (15 %) for Pseudomonas aeruginosa was caused by 0.5 J/cm(2) radiation dose and 50 μmol/L TMPyP concentration. Escherichia coli proved to be PDT resistant as the bacterial viability was higher than 90%. CONCLUSIONS: The goal of the present study was to test the efficiency of photosensitizers on the NIH 3T3 cell line and bacterial cells. Subsequently we would like to study effectiveness of photosensitizers bound to carriers (for example cyclodextrins) on other cell line and bacterial strain.

Článek

The application of antimicrobial photodynamic therapy on S. aureus and E. coli using porphyrin photosensitizers bound to cyclodextrin

Microbiological research. 2013 ; 169 (2-3) : 163-70.

Microbiol Res
ISSN 1618-0623
Medvik
Zdroj

Photodynamic therapy is usually used against malignant and non-malignant tumors. Nowadays, due to resistance of bacterial strains, we are looking for a new antimicrobial strategy to destroy bacteria with minimal invasive consequences. The worldwide increase in antibiotic resistance among different classes of gram-positive and gram-negative bacteria has led to the search for alternative anti-microbial therapies such as antimicrobial PDT (aPDT). Development antimicrobial technology combines a nontoxic compound, called photosensitizer, visible light of the appropriate wavelength, and the generation of reactive oxygen species. In this work, the photosensitizers TMPyP and ZnTPPS4 are investigated for photodynamic and antimicrobial photodynamic therapy. We tested these two porphyrins on two cell lines and two bacterial strains to compare effectiveness. In addition, we applied photosensitizers bound in the complex created with hp-β-cyclodextrin. The light-emitting diodes were used at the doses 0, 1, 5, 10 J/cm(2) for cells and 0, 150 J/cm(2) for bacteria. Tested concentrations for cells and microbes were from 0.5 to 50 μM and from 0.78 to 100 μM, respectively. From this work it can be concluded that TMPyP is a promising compound both in aPDT and in PDT, particularly in contrast to ZnTPPS4, which was efficient only in PDT. Furthermore, the eradication of gram-positive bacteria is possible only with higher concentrations of ZnTPPS4.