Kampylobakterie jsou běžně se vyskytující mikroorganismy, způsobující infekce u člověka a zvířat. Enteritis způsobená kampylobakteriemi se projevuje průjmy, horečkami a bolestmi břicha. Ačkoliv onemocnění obvykle nevyžaduje lékařské ošetření, infekce může vést k závažným zdravotním následkům, jako je reaktivní arthritida a Guillain-Barré syndrom. Pathogen se běžně vyskytuje v životním prostředí a potravinách, jak rostlinného tak živočišného původu. Tento nástin je zaměřen na současné metody isolace, identifikace a charakterisace klinicky významných kampylobakterií.
Campylobacters are ubiquitous microorganisms responsible for infections in humans and animals. An enteritis caused by campylobacters is characterized by diarrhoea, fever and abdominal pain. Although, it is usually self-limiting disease that does not require antimicrobial therapy, the infeďion can rarely lead to the serious sequeale such as reactive arthritis and Guillain-Barré syndrome. The pathogens are frequently found in an environment and many foods, of both animal and plant origin. This outline is focused on recent methods used for isolation, identification and characterization of clinically important Campylobacter species.
Campylobacters are ubiquitous microorganisms responsible for infections in humans and animals. The enteritis caused by these bacteria is characterized by diarrhoea, fever and abdominal pain. Although it is usually a self-limiting disease that does not require antimicrobial therapy, the infection can lead, though rarely, to serious sequelae such as reactive arthritis and the Guillain-Barré syndrome. The pathogens are frequently found in the environment and in many foods of both animal and plant origin. This review is focused on recent methods used for isolation, identification and characterization of clinically important Campylobacter species.
- MeSH
- bakteriologické techniky metody MeSH
- Campylobacter genetika izolace a purifikace patogenita MeSH
- diagnostické techniky molekulární MeSH
- financování organizované MeSH
- imunochemie metody MeSH
- kampylobakterové infekce epidemiologie MeSH
- polymerázová řetězová reakce metody MeSH
- reagenční diagnostické soupravy využití MeSH
- sérotypizace MeSH
Rabbit IgG raised against whole cells of Yersinia enterocolitica O:3, O:9 and against a group of pathogenic Y. enterocolitica strains (serotypes O:3, O:5,27, O:8. and O:9) were prepared. The antibody limiting titers were within the range of 1:9.5 x 10(4)-1:7.5 x 10(5). The immunoblotting analysis of Yersinia lipopolysacchides separated by SDS-PAGE showed that IgG against the single serotype O:3 interacted with high-molar-mass LPS of O:3 whereas other antibodies were bound to low-molar-mass LPS of serotypes O:3, O:5,27, O:9 and strain Y. enterocolitica (CNCTC Y 2/68). IgG against the group of pathogenic serotypes also weakly interacted with low-molar-mass LPS of serotypes O:5, O:6,30, and O:10. The cross-reactivity of the antibodies with Y. pseudotuberculosis Ia and/or Y. rohdei b, d, e, f, i, which was observed by means of dot-blotting procedure using the whole bacterial cells as an antigen, was shown not to be caused by LPS of these bacteria. The prepared antibodies were used in the development of indirect competitive ELISA. At the optimum concentration of the immunoreactants the detection limits were within the range of 3-7 x 10(6) colony-forming units per mL.
- MeSH
- chromatografie afinitní metody využití MeSH
- elektroforéza v polyakrylamidovém gelu metody využití MeSH
- ELISA metody využití MeSH
- finanční podpora výzkumu jako téma MeSH
- imunoblotting metody využití MeSH
- imunoglobulin G imunologie izolace a purifikace MeSH
- králíci imunologie MeSH
- sérový albumin hovězí imunologie MeSH
- Yersinia enterocolitica imunologie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- králíci imunologie MeSH
- zvířata MeSH
