AIM: The purpose of this study was to prepare a coladerm-beta glucan membrane (CBGM) and to evaluate its biocompatibility, cytotoxicity, antimicrobial activity, genotoxicity and mutagenicity. METHODS: The biocompatibility of the membrane was studied on the base of cell adhesion and colonization of human fibroblasts on the biomaterial surface by light microscopy. The MTT test and LDH level determination in the culture medium removed from the control and cells treated on the membrane, were used for viability and cytotoxic evaluations. Flow cytometry and gel electrophoresis were used for analysis of cell cycle and death. The antimicrobial activity of CBGM was tested using the qualitative dilution method. Ames bacteria gene mutation test and Comet assay were used for mutagenic and genotoxic studies. RESULTS: MTT and LDH tests confirmed that CBGM is a non-toxic biomaterial. Flow cytometry and gel electrophoresis demonstrated that the membrane did not affect the cell cycle and did not induce either necrotic or apoptotic cell death. CBGM exhibited antibacterial activity against G(-) bacteria E. sakazakii, S. marcescens, E. coli and agains G(+) sporogenic bacteria B. cereus. No antifungal activity was detected. The membrane did not induce mutagenicity in the bacterial reverse mutation test in Salmonella Typhimurium strains. Similarly, the comet assay showed that the tested fibroblast cells growing with/without the membrane did not show any statistically significant DNA damage. CONCLUSIONS: The CBGM has good biocompatibility, no cytotoxicity/genotoxicity/mutagenicity and it can be included as a potential scaffold for tissue engineering.
- MeSH
- antiinfekční látky farmakologie MeSH
- Bacillus subtilis růst a vývoj účinky léků MeSH
- Candida tropicalis růst a vývoj účinky léků MeSH
- chinazoliny farmakologie MeSH
- finanční podpora výzkumu jako téma MeSH
- Staphylococcus aureus růst a vývoj účinky léků MeSH
- vztahy mezi strukturou a aktivitou MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- Publikační typ
- abstrakty MeSH
Študoval sa cytotoxický a genotoxický účinok in vitro konzervačných látok pre kozmetické prostnedky JMAC TD, Bronopol, CA 24 a Euxyl K100. Z porovnania hodnôt IC100 vyplynulo, že najvyšší cytotoxický účinok na proliferáciu fibroblastových buniek V79 a VHIO indukoval Bronopol, ktorého hodnota IC100 bola v priebehu celého pokusu 10 mgA. Konzervačné látky CA 24 a Euxyl KlOO boli 4krát, resp. 5krát menej cytotoxické ako Bronopol (ICioo = 42, resp. 50,3 mgA). Kompozitná konzervačná látka na báze strieborných iónov JMAC TD prejavila najnižšiu cytotoxicitu (IC100 = 150 mgA); 15krát nižšiu ako Bronopol, 3,5krát nižšiu ako CA 24 a 3krát nižšiu ako Euxyl K100. Biocíd JMAC TD neprejavil na bakteriálnych kmeňoch Salmonella typhimurium TA 98 a TA 100 žiadnu mutagénnu aktivitu.
Cytotoxic effects of the preservative compounds for cosmetics JMAC TD, Bronopol, CA 24, and Euxyl K100 esere studied. Bronopol demonstrated the highest Cyotoxic effect on the proliferation of V79 and VH10 fibroblast cell unes - the ICioo values being 10 mg/1 during the whole experiment. The preservatives CA 24 and Euxyl K100 showed 4-times and 5-times smaller Cyotoxic activity than Bronopol IC100 = 42 or 50,3 mg/1). The preservative compounds on silver chloride ions JMAC TD manifested the lowest cytotoxicity of the preservatives tested (IC100 = 150 mg/1); 15-times smaller than Bronopol, 3,5-times smaller than CA 24 and 3-times smaller than Euxyl K100. The biocide JMAC TD did not exhibit mutagenic effects on the bacteria Salmonella typhimurium TA 98 and TA 100.
Študoval sa antibakteriálny a protinádorový účinok in vitro deviatich 2,4-disubstituovaných6H-5,1,3-benzotiadiazocínov. Žiaden z nich neprejavil v porovnaní s ampicilínom výraznú antibak-teriálnu aktivitu. Zo štúdia protinádorového účinku vyplynulo, že cytotoxicky najúčinnejšie derivátymali v polohe 2 substituent fenyl, resp. morfolín a v polohe 4 benzimidazol, imidazol, resp.1,2,4-triazol. Najvyššie testované koncentrácie derivátov I, VII a IX vyvolali okamžitý cytotoxickýúčinok, ktorý sa prejavil po 24 h kultivácii čiastočnou alebo úplnou degeneráciou nádorových buniek.Koncentrácia 30,5 mmol/L derivátu VII a koncentrácia 13,3 m mol/L derivátu IX indukovali onesko-rený cytotoxický účinok na bunky L1210. Kým po 24 a 48 h kultivácie časť bunkovej populácieproliferovala, po 72 h sa zistilo takmer úplné zastavenie bunkového delenia. Cytotoxická koncen-trácia derivátu IX porušila integritu cytoplazmovej membrány. Všetky tri vybrané benzotiadiazo-cíny (I, VII a IX) indukovali v priebehu 72 h kultivácie dvojfázový nevyvážený rast buniek HeLa.Na základe získaných výsledkov ich možno zaradiť medzi potenciálne protinádorové látky vhodnépre ďalší výskum.
Nine 2,4-disubstituted 6H-5,1,3-benzothiadiazocines have been tested for antibacterial and cytoto-xic efficacy. None of the tested compounds showed any significant antibacterial activity in compa-rison with ampicillin. The most cytotoxic effect was manifested by those derivatives which havemorpholine or phenyl in position 2 and benzimidazole, imidazole or 1,2,4-triazole in position 4. Thehighest concentrations of derivatives I, VII, IX induced an acute cytotoxic effect which wasmanifested by cell lysis after 24 h of culturing. Derivative VII, concentration 30.5 mmol/L, andderivative IX, concentration 13.3 mmol/L, exhibited a delayed cytotoxic effect on L1210 cells. Whileduring the first 24 and 48 h a certain part of cell population proliferated, during further 24 h a totalinhibition of cell division was found. The cytotoxic concentration of derivative IX induced damageto the integrity of the cytoplasmic membrane. Benzothiadiazocines I, VII and IX induced a two-phaseunbalanced growth. Based on these results, benzothiodiazocines I, VII and IX could be considerednew potential anticancer drugs which are appropriate for further research.
