- MeSH
- asistovaná reprodukce klasifikace MeSH
- fertilizace in vitro klasifikace MeSH
- infertilita * terapie MeSH
- lidé MeSH
- preimplantační diagnóza klasifikace MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- přehledy MeSH
Together with auxins, cytokinins are the main plant hormones involved in many different physiological processes. Given this knowledge, cytokinin levels can be manipulated by genetic modification in order to improve agronomic parameters of cereals in relation to, for example, morphology, yield, and tolerance to various stresses. The barley (Hordeum vulgare) cultivar Golden Promise was transformed using the cytokinin dehydrogenase 1 gene from Arabidopsis thaliana (AtCKX1) under the control of mild root-specific β-glucosidase promoter from maize. Increased cytokinin degradation activity was observed positively to affect the number and length of lateral roots. The impact on morphology depended upon the recombinant protein's subcellular compartmentation. While assumed cytosolic and vacuolar targeting of AtCKX1 had negligible effect on shoot growth, secretion of AtCKX1 protein to the apoplast had a negative effect on development of the aerial part and yield. Upon the application of severe drought stress, all transgenic genotypes maintained higher water content and showed better growth and yield parameters during revitalization. Higher tolerance to drought stress was most caused by altered root morphology resulting in better dehydration avoidance.
- MeSH
- aklimatizace genetika fyziologie MeSH
- biotechnologie MeSH
- cytokininy genetika metabolismus MeSH
- fenotyp MeSH
- fyziologický stres MeSH
- geneticky modifikované rostliny MeSH
- ječmen (rod) genetika růst a vývoj fyziologie MeSH
- kořeny rostlin fyziologie MeSH
- metabolické sítě a dráhy MeSH
- období sucha MeSH
- oxidoreduktasy genetika MeSH
- proteiny huseníčku genetika fyziologie MeSH
- regulátory růstu rostlin genetika metabolismus MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- rostlinné geny MeSH
- rostlinné proteiny genetika MeSH
- stanovení celkové genové exprese MeSH
- upregulace MeSH
- Publikační typ
- časopisecké články MeSH
