Antibiotic resistance (ATBR) is increasing every year as the overuse of antibiotics (ATBs) and the lack of newly emerging antimicrobial agents lead to an efficient pathogen escape from ATBs action. This trend is alarming and the World Health Organization warned in 2021 that ATBR could become the leading cause of death worldwide by 2050. The development of novel ATBs is not fast enough considering the situation, and alternative strategies are therefore urgently required. One such alternative may be the use of non-thermal plasma (NTP), a well-established antimicrobial agent actively used in a growing number of medical fields. Despite its efficiency, NTP alone is not always sufficient to completely eliminate pathogens. However, NTP combined with ATBs is more potent and evidence has been emerging over the last few years proving this is a robust and highly effective strategy to fight resistant pathogens. This minireview summarizes experimental research addressing the potential of the NTP-ATBs combination, particularly for inhibiting planktonic and biofilm growth and treating infections in mouse models caused by methicillin-resistant Staphylococcus aureus or Pseudomonas aeruginosa. The published studies highlight this combination as a promising solution to emerging ATBR, and further research is therefore highly desirable.
- MeSH
- antibakteriální látky * farmakologie terapeutické užití MeSH
- antibiotická rezistence MeSH
- bakteriální léková rezistence MeSH
- biofilmy * účinky léků MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků MeSH
- modely nemocí na zvířatech MeSH
- myši MeSH
- plazmové plyny * farmakologie MeSH
- pseudomonádové infekce mikrobiologie farmakoterapie MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- stafylokokové infekce mikrobiologie farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
The emergence of antibiotic resistance in opportunistic pathogens represents a huge problem, the solution for which may be a treatment with a combination of multiple antimicrobial agents. Sodium salt of cobalt bis-dicarbollide (COSAN.Na) is one of the very stable, low-toxic, amphiphilic boron-rich sandwich complex heteroboranes. This compound has a wide range of potential applications in the biological sciences due to its antitumor, anti-HIV-1, antimicrobial and antibiofilm activity. Our study confirmed the ability of COSAN.Na (in the concentration range 0.2-2.48 μg/mL) to enhance tetracycline, erythromycin, and vancomycin action towards Staphylococcus epidermidis planktonic growth with an additive or synergistic effect (e.g., the combination of 1.24 μg/mL COSAN.Na and 6.5 μg/mL TET). The effective inhibitory concentration of antibiotics was reduced up to tenfold most efficiently in the case of tetracycline (from 65 to 6.5 μg/mL). In addition, strong effect of COSAN.Na on disruption of the cell envelopes was determined using propidium iodide uptake measurement and further confirmed by transmission electron microscopy. The combination of amphiphilic COSAN.Na with antibiotics can therefore be considered a promising way to overcome antibiotic resistance in Gram-positive cocci.
- Publikační typ
- časopisecké články MeSH
Candida albicans has several virulence factors at its disposal, including yeast-hyphal transition associated with biofilm formation, phospholipases, proteases and hemolytic activity, all of which contribute to its pathogenesis. We used synthetic derivative LL-III/43 of antimicrobial peptide lasioglossin LL-III to enhance effect of azoles on attenuation of C. albicans virulence factors. LL-III/43 was able to inhibit initial adhesion or biofilm formation of C. albicans strains at 50 µM. Azoles, however, were ineffective at this concentration. Using fluorescently labeled LL-III/43, we observed that peptide covered C. albicans cells, partially penetrated through their membranes and then accumulated inside cells. LL-III/43 (25 µM) in combination with clotrimazole prevented biofilm formation already at 3.1 µM clotrimazole. Neither LL-III/43 nor azoles were able to significantly inhibit phospholipases, proteases, or hemolytic activity of C. albicans. LL-III/43 (25 µM) and clotrimazole (50 µM) in combination decreased production of these virulence factors, and it completely attenuated its hemolytic activity. Scanning electron microscopy showed that LL-III/43 (50 µM) prevented C. albicans biofilm formation on Ti-6Al-4 V alloy used in orthopedic surgeries and combination of LL-III/43 (25 µM) with clotrimazole (3.1 µM) prevented biofilm formation on urinary catheters. Therefore, mixture of LL-III/43 and clotrimazole is suitable candidate for future pharmaceutical research.
- MeSH
- antifungální látky farmakologie MeSH
- azoly farmakologie MeSH
- biofilmy účinky léků růst a vývoj MeSH
- Candida albicans účinky léků MeSH
- erytrocyty účinky léků MeSH
- faktory virulence MeSH
- fosfolipasy antagonisté a inhibitory MeSH
- hemolýza účinky léků MeSH
- hydrofobní a hydrofilní interakce účinky léků MeSH
- kationické antimikrobiální peptidy chemická syntéza farmakokinetika MeSH
- lidé MeSH
- proteasy metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Klíčová slova
- Mithridat, electuarium, theriak,
- MeSH
- dějiny 18. století MeSH
- dějiny lékárnictví MeSH
- farmacie MeSH
- léčivé přípravky dějiny MeSH
- příprava léků dějiny MeSH
- Check Tag
- dějiny 18. století MeSH
- Publikační typ
- historické články MeSH
- Klíčová slova
- electuarium, theriak, Mithridat,
- MeSH
- dějiny lékárnictví MeSH
- dějiny novověku 1601- MeSH
- dějiny starověku MeSH
- farmacie MeSH
- jedy terapeutické užití MeSH
- léčivé přípravky * dějiny MeSH
- lidé MeSH
- Check Tag
- dějiny starověku MeSH
- lidé MeSH
- Publikační typ
- historické články MeSH
- Klíčová slova
- Mithridat, theriak, electuarium,
- MeSH
- dějiny 18. století MeSH
- dějiny lékárnictví MeSH
- farmacie MeSH
- jeptišky * dějiny MeSH
- léčivé přípravky * dějiny MeSH
- lidé MeSH
- Check Tag
- dějiny 18. století MeSH
- lidé MeSH
- Publikační typ
- historické články MeSH
- Klíčová slova
- Mithridat, theriak, electuarium,
- MeSH
- dějiny 18. století MeSH
- dějiny 19. století MeSH
- dějiny lékárnictví MeSH
- farmacie MeSH
- jedy terapeutické užití MeSH
- léčivé přípravky * dějiny MeSH
- lidé MeSH
- Check Tag
- dějiny 18. století MeSH
- dějiny 19. století MeSH
- lidé MeSH
- Publikační typ
- historické články MeSH
- MeSH
- farmakologie MeSH
- lidé MeSH
- reklama zaměřená na spotřebitele * zákonodárství a právo MeSH
- Check Tag
- lidé MeSH
