- MeSH
- imunitní systém MeSH
- nádory rekta imunologie MeSH
- nádory tračníku imunologie MeSH
- nádory imunologie MeSH
- výzkum MeSH
- Publikační typ
- rozhovory MeSH
New foods and natural biological modulators have recently become of scientific interest in the investigation of the value of traditional medical therapeutics. Glucans have an important part in this renewed interest. These fungal wall components are claimed to be useful for various medical purposes and they are obtained from medicinal mushrooms commonly used in traditional Oriental medicine. The immunotherapeutic properties of fungi extracts have been reported, including the enhancement of anticancer immunity responses. These properties are principally related to the stimulation of cells of the innate immune system. The discovery of specific receptors for glucans on dendritic cells (dectin-1), as well as interactions with other receptors, mainly expressed by innate immune cells (e.g., Toll-like receptors, complement receptor-3), have raised new attention toward these products as suitable therapeutic agents. We briefly review the characteristics of the glucans from mycelial walls as modulators of the immunity and their possible use as antitumor treatments.
- MeSH
- adjuvancia imunologická chemie terapeutické užití MeSH
- Agaricales chemie MeSH
- beta-glukany chemie terapeutické užití MeSH
- buněčné extrakty terapeutické užití MeSH
- dendritické buňky účinky léků MeSH
- lektiny typu C metabolismus MeSH
- lidé MeSH
- makrofágy účinky léků imunologie MeSH
- myši MeSH
- přirozená imunita účinky léků MeSH
- protinádorové látky chemie imunologie terapeutické užití MeSH
- signální transdukce imunologie MeSH
- tradiční orientální medicína MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
BACKGROUND: Nanoparticle-based systems are promising for the development of imaging and therapeutic agents. The main advantage of nanoparticles over traditional systems lies in the possibility of loading multiple functionalities onto a single molecule, which are useful for therapeutic and/or diagnostic purposes. These functionalities include targeting moieties which are able to recognize receptors overexpressed by specific cells and tissues. However, targeted delivery of nanoparticles requires an accurate system design. We present here a rationally designed, genetically engineered, and chemically modified protein-based nanoplatform for cell/tissue-specific targeting. METHODS: Our nanoparticle constructs were based on the heavy chain of the human protein ferritin (HFt), a highly symmetrical assembly of 24 subunits enclosing a hollow cavity. HFt-based nanoparticles were produced using both genetic engineering and chemical functionalization methods to impart several functionalities, ie, the α-melanocyte-stimulating hormone peptide as a melanoma-targeting moiety, stabilizing and HFt-masking polyethylene glycol molecules, rhodamine fluorophores, and magnetic resonance imaging agents. The constructs produced were extensively characterized by a number of physicochemical techniques, and assayed for selective melanoma-targeting in vitro and in vivo. RESULTS: Our HFt-based nanoparticle constructs functionalized with the α-melanocyte-stimulating hormone peptide moiety and polyethylene glycol molecules were specifically taken up by melanoma cells but not by other cancer cell types in vitro. Moreover, experiments in melanoma-bearing mice indicate that these constructs have an excellent tumor-targeting profile and a long circulation time in vivo. CONCLUSION: By masking human HFt with polyethylene glycol and targeting it with an α-melanocyte-stimulating hormone peptide, we developed an HFt-based melanoma-targeting nanoplatform for application in melanoma diagnosis and treatment. These results could be of general interest, because the same strategy can be exploited to develop ad hoc nanoplatforms for specific delivery towards any cell/tissue type for which a suitable targeting moiety is available.
- MeSH
- alfa-MSH chemie diagnostické užití MeSH
- apoferritiny chemie MeSH
- buňky HT-29 MeSH
- fluorescenční barviva chemie diagnostické užití MeSH
- konfokální mikroskopie MeSH
- lékové transportní systémy MeSH
- lidé MeSH
- magnetická rezonanční tomografie MeSH
- magnetické nanočástice chemie diagnostické užití ultrastruktura MeSH
- melanom experimentální diagnóza MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- nanomedicína MeSH
- nanotechnologie MeSH
- polyethylenglykoly chemie MeSH
- rekombinantní proteiny chemie MeSH
- stabilita proteinů MeSH
- transmisní elektronová mikroskopie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The recurrence of a cancer - local or distant (metastasis) - is manifested by the persistence of cancer cells in the organism after the ablation of the primary lesion, an ineffective anticancer immune response, and by the activity of biological/immunological factors that can stimulate and sustain its development. This review focuses on colorectal carcinoma and discusses some aspects of cancer immunology regarding cancer development and its recurrence. It is addressed also to the clinician to provide new insights helpful for designing better therapeutic strategies and patient's follow up. Therapeutic approaches used during and after surgical treatments, found capable of modulating immunity (differently affecting disease outcome), will also be described.
- MeSH
- imunoterapie metody MeSH
- kolorektální nádory imunologie patofyziologie terapie MeSH
- lidé MeSH
- lokální recidiva nádoru imunologie prevence a kontrola MeSH
- metastázy nádorů imunologie prevence a kontrola MeSH
- NF-kappa B metabolismus MeSH
- transkripční faktor STAT3 metabolismus MeSH
- zánět imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
