- MeSH
- antikonvulziva aplikace a dávkování MeSH
- cílená molekulární terapie metody trendy MeSH
- diagnostické techniky neurologické trendy MeSH
- elektrická stimulace přístrojové vybavení MeSH
- elektroencefalografie trendy MeSH
- epilepsie * diagnóza terapie MeSH
- individualizovaná medicína metody trendy MeSH
- lidé MeSH
- neurologie * trendy MeSH
- přehodnocení terapeutických indikací léčivého přípravku trendy MeSH
- transplantace trendy MeSH
- umělá inteligence MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Jak diagnostikovat epilepsii po cévní mozkové příhodě? Jaká jsou rizika jejího rozvoje? A konečně kdy, jak a čím tuto epilepsii léčit a na co si dát při léčbě pozor? Bez znalostí odpovědí na tyto otázky nelze toto onemocnění s narůstající prevalencí efektivně léčit. Nevhodná diagnostika a léčba naopak může vést ke stigmatizaci pacientů a k závažným nežádoucím účinkům. Odpovědět na tyto a mnohé další otázky si dává za úkol tento přehled týkající se onemocnění, které přináší pacientům mnohá rizika.
How should be the post-stroke epilepsy diagnosed? What are the risks of developping this condition after the stroke? And finally, how should we treat this condition and what are the tricky aspects of it? Without this knowledge, it is impossible to treat post-stroke epilepsy effectively. Inappropriate treatment can bring unnecessary patient stigmatization and serious side effects. This review aimes to provide answers to these questions connected to the disease with raising prevalence bringing many risk to the patients.
- MeSH
- cévní mozková příhoda * diagnóza komplikace MeSH
- diferenciální diagnóza MeSH
- elektroencefalografie MeSH
- epilepsie * diagnóza farmakoterapie MeSH
- lidé MeSH
- rizikové faktory MeSH
- záchvaty epidemiologie etiologie farmakoterapie prevence a kontrola MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
The phenomenon of déjà vu (DV) has intrigued scientists for decades, yet its neurophysiological underpinnings remain elusive. Brain regions have been identified in which morphometry differs between healthy individuals according to the frequency of their DV experiences. This study built upon these findings by assessing if and how neural activity in these and other brain regions also differ with respect to DV experience. Resting-state fMRI was performed on 68 healthy volunteers, 44 of whom reported DV experiences (DV group) and 24 who did not (NDV group). Using multivariate analyses, we then assessed the (fractional) amplitude of low-frequency fluctuations (fALFF/ALFF), a metric that is believed to index brain tissue excitability, for five discrete frequency bands within sets of brain regions implicated in DV and those comprising the default mode network (DMN). Analyses revealed significantly lower values of fALFF/ALFF for specific frequency bands in the DV relative to the NDV group, particularly within mesiotemporal structures, bilateral putamina, right caudatum, bilateral superior frontal cortices, left lateral parietal cortex, dorsal and ventral medial prefrontal cortex, and the posterior cingulate cortex. The pattern of differences in fALFF/ALFF measures between the brains of individuals who have experienced DV and those who have not provides new neurophysiological insights into this phenomenon, including the potential role of the DMN. We suggest that the erroneous feeling of familiarity arises from a temporary disruption of cortico-subcortical circuitry together with the upregulation of cortical excitability.
- MeSH
- emoce MeSH
- lidé MeSH
- magnetická rezonanční tomografie * metody MeSH
- mapování mozku metody MeSH
- mozek diagnostické zobrazování MeSH
- mozkové vlny * fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- dějiny 20. století MeSH
- přenos infekční nemoci dějiny MeSH
- vozhřivka * dějiny MeSH
- zoonózy dějiny MeSH
- Check Tag
- dějiny 20. století MeSH
- Publikační typ
- biografie MeSH
- historické články MeSH
- O autorovi
- Derbek, Miroslav Autorita
A method for the immobilization of antibodies to inert matrix represents an important factor that affects results of immunoaffinity chromatography. Binding antibodies to immobilized metal ions is an example of oriented immobilization that avoids a random coupling of a protein. Preparation of a stable immunoaffinity sorbent using immobilized metal ions was described. Antibodies were bound to chelated Co3+ ions that were prepared by oxidation of Co2+-iminodiacetic acid agarose using hydrogen peroxide. The formation of a stable complex of the antibody with immobilized Co3+ ions was proved. Antibodies bound by this way were not released with solutions of 50 mM EDTA, 6 M urea, 3 M NaCl, 20% v/v dioxane, 0.1 M imidazole and buffers of pH 2.5 and pH 11.0. If needed, antibody could be released from the carrier by the reduction of Co3+ ions with a reducing agent (e.g. dithiotreitol or 2-mercaptoethanol). Antibody released from the carrier could be then replaced by another antibody. The method described in this paper was used for the immobilization of polyclonal rabbit anti-ovalbumin antibody or egg yolk antibody (IgY) produced in chicken. In a model experiment, immobilized polyclonal rabbit antibodies were used for the separation of ovalbumin from egg white and conditions of chromatography were described.
- MeSH
- chromatografie afinitní metody MeSH
- financování organizované MeSH
- imunoglobulin G chemie metabolismus MeSH
- imunoglobuliny chemie metabolismus MeSH
- kobalt chemie MeSH
- králíci MeSH
- kur domácí MeSH
- oxidancia chemie MeSH
- peroxid vodíku chemie MeSH
- protilátky chemie metabolismus MeSH
- sefarosa chemie MeSH
- vaječné proteiny chemie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- hodnotící studie MeSH
