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Submerged cultures of the low-production strain of Tolypocladium inflatum DSM 63544 formed a mixture of cyclosporins (CS) consisting of CS-A, CS-B, "CS-3" and "CS-4". Glucose, sucrose and maltose were highly favored for biomass production but provided a different physiological state necessary for CS biosynthesis. Not only the magnitude of CS production but also the proportion of individual components of the CS mixture were affected by the C source. Intensive CS synthesis was in correlation with the formation of CS-3. Lower yields of CS were accompanied by an increased proportion of CS-A in the CS complex. The best specific production of CS was achieved on the glucose medium, the highest yield of CS-A on the maltose medium. There was no remarkable relationship between the biomass formation and the intensity of CS synthesis.

MeSH
cyklosporiny biosyntéza chemie MeSH
kinetika MeSH
kultivační média MeSH
mitosporické houby růst a vývoj metabolismus MeSH
molekulární sekvence - údaje MeSH
molekulární struktura MeSH
sekvence aminokyselin MeSH
Publikační typ
časopisecké články MeSH

Článek

Physiological controls and regulation of ergot alkaloid formation

Řeháček, Zdeněk, 1925-2002
Autor Autorita Institute of Microbiology, Czechoslovak Academy of Sciences, Prague

Folia microbiologica. 1991 ; 36 (4) : 323-342.

Folia microbiol. (Prague)
ISSN 0015-5632 (tištěná verze)
Medvik
Zdroj

Innovation and technical development of ergot alkaloids (EA) has moved closer to scientific research. Circumstantial evidence presently links the initiation of EA metabolism to changes in a range of parameters--morphology, concentrations of enzymes and their substrates, nutrients and external stress. The biosynthesis of EA begins at the level of the genetic information apparatus and continues at the level of physiological expression. EA and their formation play a role in the physiology of the production organism. Insufficient insight into Claviceps physiology hampers the deployment of computers in the control and regulation of the EA process. Knowledge of physiological controls and genetic manipulation are the principal tools of modern EA production. In principle it is now possible to improve EA yields by a concerted breeding of the ergot fungus by sexual and parasexual genetic engineering.