Proteomika je stále viac používanou metódou vo viacerých odvetviach medicíny, avšak jej aplikácia v psychiatrii je stále ve3⁄4mi obmed-zená. V našej pilotnej štúdii sme identifikovali alterovanú skupinu proteínov s použitím proteomickej analýzy mozgovomiechovéhomoku a krvných doštièiek u suicidálnych jedincov v rámci metabolických dráh glykolýzy/glukoneogenézy, 14-3-3 sprostredkovanej sig-nalizácie a komplementovej a koagulaènej kaskády. Na základe našich výsledkov predpokladáme, že zmeny metabolizmu glukózy spoluso zmenami komplementovej a koagulaènej kaskády a 14-3-3 sprostredkovanej signalizácie môžu hraś úlohu v neurobiológii samovrážd v zmysle zníženej utilizácie glukózy a zhoršenej odpovede na oxidatívny stres. Skupinu proteínov identifikovaných v našej pilotnej štúdii, tzv. potencionálnych kandidátov biomarkerov suicidality, je však nevyhnutné overiś ïalšími výskumami na väèšom súbore jedincov.
Despite the fact that proteomic analysis is becoming widely used in various medical fields its use in psychiatry is still very limited. We decided to study suicidal behaviour via cerebrospinal fluid and platelets with the use of proteomics. We identified a group of alte-red proteins in suicidal patients in the metabolic pathways of glycolysis/gluconeogenesis, complement and coagulation cascade and14-3-3 mediated signaling pathway. Based on these findings we suppose that alterations of glucose metabolism (especially utilisation of glucose and altered response to oxidative stress) together with alterations in complement and coagulation cascade and 14-3-3 mediated signaling pathway may play a role in the neurobiology of suicide. However, further research is needed to clarify whether the identified group of proteins can be used as a potential peripheral biomarker for suicidal behaviour.
- MeSH
- biologické markery krev MeSH
- glukoneogeneze MeSH
- glykolýza MeSH
- hemokoagulace MeSH
- komplement MeSH
- lidé středního věku MeSH
- lidé MeSH
- pilotní projekty MeSH
- pitva MeSH
- proteiny 14-3-3 MeSH
- proteiny v mozkomíšním moku * MeSH
- proteiny izolace a purifikace klasifikace MeSH
- proteomika * MeSH
- sebevražda * MeSH
- trombocyty * chemie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
We investigated whether early postnatal over-nutrition affects normal course of skin wound healing. To induce over-nutrition the litter size was adjusted on the first day after birth to four pups/nest (small litters). In parallel, as a control, normal nests of 10 pups/nest (normal litters) were used. For the wound healing experiment 30 male Sprague-Dawley rats, 15 from normal nests and 15 from small nests, were used. Two parallel full-thickness skin incisions and two full-thickness excisions were performed on the back of each rat. Samples for histological examination (excisions) and wound tensile strength measurement (incisions) were collected on days 2, 6, and 14 after surgery. Our study demonstrates that rats from the small nests had enhanced plasma levels of insulin and enhanced body weight/fat parameters. Furthermore, in small nests, rats that expressed the above-mentioned symptoms displayed slight improvement of epidermis regeneration, accelerated demarcation line formation, and increased wound tensile strength. From this point of view the small nest model used in the present experiment is helpful for exploration whether these acquired changes might be considered as a sufficient essential factor involved in the regulation of metabolic homeostasis and wound repair in juvenile obese male rats. Nevertheless, further studies need to be performed to verify the present findings also on other animal models and humans and to describe the exact underlying mechanism.
- MeSH
- hojení ran * MeSH
- homeostáza MeSH
- inzulin metabolismus MeSH
- krysa rodu rattus MeSH
- kůže patologie MeSH
- metabolický syndrom genetika MeSH
- modely nemocí na zvířatech MeSH
- novorozená zvířata MeSH
- obezita genetika MeSH
- odstavení MeSH
- pevnost v tahu MeSH
- potkani Sprague-Dawley MeSH
- tělesná hmotnost MeSH
- věda o výživě zvířat MeSH
- velikost vrhu MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The in vivo antitumour activity of the natural photosensitizer hypericin was evaluated. C3H/DiSn mice were inoculated with fibrosarcoma G5:1:13 cells. When the tumour reached a volume of 40-80mm3 the mice were intraperitoneally injected with hypericin, either in a single dose (5mg/kg; 1 or 6h before laser irradiation) or two fractionated doses (2.5 mg/kg; 6 and 1 h before irradiation with laser light; 532 nm, 70mW/cm2, 168 J/cm2). All tumours in control groups treated with hypericin alone as well as those irradiated with laser light alone had similar growth rates and none of these tumours regressed spontaneously. Complete remission of tumour in photodynamic therapy (PDT)-treated groups was similar (14-17% single dose vs. 33% fractionated dose), but the fractionated schedule of hypericin dosing was found to be more efficient than the single dose, measured by survival assay (p < 0.05). Our experimental model showed that fractionated administration of hypericin can produce a better therapeutic response than single administration
- MeSH
- antitumorózní látky fytogenní aplikace a dávkování farmakologie terapeutické užití MeSH
- fibrosarkom farmakoterapie MeSH
- financování vládou MeSH
- fotochemoterapie MeSH
- fotosenzibilizující látky aplikace a dávkování farmakologie terapeutické užití MeSH
- fytoterapie MeSH
- inbrední kmeny myší MeSH
- injekce intraperitoneální MeSH
- perylen analogy a deriváty aplikace a dávkování farmakologie terapeutické užití MeSH
- rozvrh dávkování léků MeSH
- světlo MeSH
- třezalka MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
