This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17beta-estradiol and progesterone, and human transforming growth factor-beta2 (TGF-beta2) and TGF-beta2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 microg.ml-1. It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17beta-estradiol, progesterone, TGF-beta2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 microg.ml-1, which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 microg.ml-1) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells.
- MeSH
- aneuploidie MeSH
- cytogenetické vyšetření metody statistika a číselné údaje MeSH
- exprese genu MeSH
- faktor VIII genetika MeSH
- femur anatomie a histologie MeSH
- finanční podpora výzkumu jako téma MeSH
- geneticky modifikovaná zvířata anatomie a histologie genetika MeSH
- Haversův systém anatomie a histologie MeSH
- králíci MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
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- buněčné jádro ultrastruktura MeSH
- epidermální růstový faktor farmakologie MeSH
- folikulární buňky cytologie fyziologie účinky léků MeSH
- králíci MeSH
- mitogenem aktivované proteinkinasy metabolismus MeSH
- proliferační antigen buněčného jádra analýza MeSH
- steroidy metabolismus MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- ženské pohlaví MeSH
- zvířata MeSH
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- embryo savčí MeSH
- embryonální vývoj MeSH
- insulinu podobný růstový faktor I fyziologie MeSH
- králíci MeSH
- mitogenem aktivované proteinkinasy fyziologie MeSH
- nukleotidy cyklické biosyntéza MeSH
- proteinkinasy závislé na cyklickém AMP fyziologie MeSH
- steroidy sekrece MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
