A large epidemic caused by shigatoxigenic Escherichia coli (E. coli) in spring 2011 in Germany resulted in reduction of trust in the health safety of raw vegetables and sprouted seeds. This study focused on the detection and characterization of E. coli in raw vegetables and sprouted seeds sold in the Czech Republic. Out of 91 samples, 24 (26.4%) were positive for the presence of E. coli. Resistance to antimicrobial agents was determined by the disk diffusion method and E-test. Polymerase chain reaction was used for the detection of selected genes encoding virulence--eaeA, hly, stx1, and stx2 and genes encoding resistance to tetracycline--tet(A), tet(B), tet(C), and tet(G) and to β-lactams--blaTEM, blaSHV, and blaCTX. The blaTEM gene was detected in two isolates, the tet(B) gene in three and tet(A) in one isolate. No hly, stx1, or stx2 genes were present, but the eaeA gene was found in three (11.1%) isolates from imported vegetables. These isolates can be considered as potentially enteropathogenic. Results of this study show that raw vegetables and sprouted seeds sold in the retail market can represent a potential risk for consumers.
- MeSH
- bakteriální geny * MeSH
- Escherichia coli genetika izolace a purifikace MeSH
- mikrobiální testy citlivosti MeSH
- polymerázová řetězová reakce MeSH
- potravinářská mikrobiologie MeSH
- proteiny z Escherichia coli genetika MeSH
- pulzní gelová elektroforéza MeSH
- rezistence na tetracyklin genetika MeSH
- semena rostlinná mikrobiologie MeSH
- virulence genetika MeSH
- zásobování potravinami MeSH
- zelenina mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
The aim of this study was to assess the quality of raw cow's milk from an automatic milking system. Samples of milk (48) were analyzed chemically and microbiologically and the somatic cell count, freezing point and inhibitor residues were determined. For comparison purposes, milk analysis data from two farms using conventional machine milking and 2008 milk analysis report data for the Czech Republic were used. All physical and chemical characteristics of the study samples were within the established limits. The average content (%) of chemical indicators was following: fat 3.79 ± 0.18, protein 3.46 ± 0.06, casein 2.67 ± 0.09, lactose 4.82 ± 0.04 and NFS 8.96 ± 0.11. The values for freezing point and somatic cells count were 221 ± 46.103·ml-1 and -0.521 ± 0.003 °C respectively. No inhibitor residue was detected in any of the samples. Microbiological values were lower than the limits: the total microbial count (2.3·104 CFU·ml-1), psychrotrophic count (6.3·103 CFU·ml-1), coliform count (2.4·101 CFU·ml-1), Escherichia coli count (3.2·101 CFU·ml-1), enterococci count (8.5·101 CFU·ml-1) and Staphylococcus aureus count (1.1·101 CFU·ml-1). The study provides a comprehensive view on the quality of milk produced by robotic milking. The automatic milking system seems to be excellent in terms of milking and milk hygiene
- Klíčová slova
- robotické dojení, somatické buňky,
- MeSH
- analýza potravin MeSH
- bezpečnost potravin * MeSH
- lidé MeSH
- mléko * chemie mikrobiologie MeSH
- tranzitní teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
