- Publikační typ
- abstrakt z konference MeSH
We tested the effect of two different concentrations (150μg/l and 0.15μg/l) of mycotoxin zearalenone (ZEA) on the reproductive parameters and expression of testicular genes in male mice. In adult males, no reduction of body or reproductive organ weight was observed, and the seminiferous tubules were morphologically normal with ongoing spermatogenesis. However, we found decreased sperm concentration, increase of morphologically abnormal spermatozoa and increased binding of apoptotic marker annexin V. This study was also focused on the evaluation of gene expression profiles of 28 genes playing important roles during the processes occurring in the testicular tissue. We detected changes in the expression of genes important for proper spermatogenesis. Surprisingly, we observed a stronger effect after exposure to the lower dose of ZEA.
- MeSH
- apoptóza účinky léků MeSH
- estrogeny nesteroidní toxicita MeSH
- exprese genu účinky léků MeSH
- myši MeSH
- spermatogeneze účinky léků MeSH
- spermie účinky léků patologie MeSH
- testis účinky léků metabolismus MeSH
- zearalenon toxicita MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Tetrabromobisphenol A (TBBPA) is the main flame retardant used in printed circuit boards and laminates. The human population is highly exposed to TBBPA as it is used in consumer electronics as well as office and communication equipment. The main use of hexabromocyclododecane (HBCD) is in insulation foam boards, which are widely used in the construction sector. Brominated flame retardants may possess endocrine disrupting activity and thus represent a threat to the environment, including humans and their reproduction. The aim of this work was to evaluate the oestrogenic effects of TBBPA and HBCD in vitro on MCF-7 cells. We used the proliferation test (E-screen assay) in MCF-7 breast cancer cells and reverse transcription quantitative polymerase chain reaction analysis of TFF1 gene expression to analyse oestrogenicity of the studied compounds. RT-qPCR has proved to be a fast and valuable molecular technique in gene expression quantification. HBCD but not TBBPA increased cell proliferation in MCF-7 cells and up-regulated TFF1 gene expression in a concentration-dependent manner. Anti-oestrogen ICI 182,780 inhibited up-regulation of TFF1 by HBCD. We have shown that HBCD displays oestrogen- like effects on MCF-7 cells. TBBPA, on the other hand, has not shown any oestrogenic effect mediated by the oestrogen receptor ?.
- MeSH
- alfa receptor estrogenů metabolismus MeSH
- antagonisté estrogenu toxicita MeSH
- bromované uhlovodíky toxicita MeSH
- endokrinní disruptory toxicita MeSH
- estriol analogy a deriváty metabolismus MeSH
- estrogeny analýza MeSH
- exprese genu MeSH
- financování organizované MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádorové supresorové proteiny metabolismus MeSH
- polybrombifenylové sloučeniny toxicita MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- proliferace buněk MeSH
- retardanty hoření toxicita MeSH
- upregulace MeSH
- Check Tag
- lidé MeSH
