Staphylococcus aureus infections present a significant threat to the global healthcare system. The increasing resistance to existing antibiotics and their limited efficacy underscores the urgent need to identify new antibacterial agents with low toxicity to effectively combat various S. aureus infections. Hence, in this study, we have screened T-muurolol for possible interactions with several S. aureus-specific bacterial proteins to establish its potential as an alternative antibacterial agent. Based on its binding affinity and interactions with amino acids, T-muurolol was identified as a potential inhibitor of S. aureus lipase, dihydrofolate reductase, penicillin-binding protein 2a, D-Ala:D-Ala ligase, and ribosome protection proteins tetracycline resistance determinant (RPP TetM), which indicates its potentiality against S. aureus and its multi-drug-resistant strains. Also, T-muurolol exhibited good antioxidant and anti-inflammatory activity by showing strong binding interactions with flavin adenine dinucleotide (FAD)-dependent nicotinamide adenine dinucleotide phosphate (NAD(P)H) oxidase, and cyclooxygenase-2. Consequently, molecular dynamics (MD) simulation and recalculating binding free energies elucidated its binding interaction stability with targeted proteins. Furthermore, quantum chemical structure analysis based on density functional theory (DFT) depicted a higher energy gap between the highest occupied molecular orbital and lowest unoccupied molecular orbital (EHOMO-LUMO) with a lower chemical potential index, and moderate electrophilicity suggests its chemical hardness and stability and less polarizability and reactivity. Additionally, pharmacological parameters based on ADMET, Lipinski's rules, and bioactivity score validated it as a promising drug candidate with high activity toward ion channel modulators, nuclear receptor ligands, and enzyme inhibitors. In conclusion, the current findings suggest T-muurolol as a promising alternative antibacterial agent that might be a potential phytochemical-based drug against S. aureus. This study also suggests further clinical research before human application.

• MeSH
• antibakteriální látky * farmakologie   chemie   MeSH
• antioxidancia farmakologie   chemie   MeSH
• bakteriální proteiny antagonisté a inhibitory   metabolismus   chemie   MeSH
• fytonutrienty * farmakologie   chemie   MeSH
• lidé MeSH
• objevování léků * metody   MeSH
• počítačová simulace MeSH
• simulace molekulární dynamiky MeSH
• simulace molekulového dockingu MeSH
• stafylokokové infekce farmakoterapie   mikrobiologie   MeSH
• Staphylococcus aureus * účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Rozhovor s Markem Dvořákem, českým lékařem letecké záchranné služby a urgentního příjmu. Určeno široké veřejnosti.

• MeSH
• dějiny 21. století MeSH
• letecká záchranná služba dějiny   MeSH
• pomocní zdravotničtí pracovníci dějiny   MeSH
• urgentní zdravotnické služby dějiny   MeSH
• Check Tag
• dějiny 21. století MeSH
• Publikační typ
• rozhovory MeSH
• Geografické názvy
• Česká republika MeSH

• Konspekt
• Patologie. Klinická medicína
• Biografie
• NLK Obory
• urgentní lékařství

• O autorovi
• Dvořák, Marek, 1985- Autorita

Emotional creativity (EC) refers to cognitive abilities and personality traits related to the originality of emotional experience and expression. Previous studies have found that the COVID-19 epidemic and the restrictions imposed increased the levels of negative emotions, which obstructed adaptation. This research suggests that EC predicts the motivation for innovative adaptive behavior under the restrictions of COVID-19. In the case study of university professors, we show that EC predicts the motivation to creatively capitalize on the imposed online teaching in looking for innovative research and personal development. Methodologically, we rely on the Emotional Creativity Inventory (ECI) administered to a sample of 463 university professors (41.5% men, aged 22-100. M ± SD = 45.53 ± 11.46, median 44) from the Czech Republic (N = 137), Slovak Republic (N = 61), and Russia (N = 265). The indicators for motivation for innovative performance included motivations to use distant methods of scientific research, to look for partners for conducting scientific research in other cities or abroad, to conduct interdisciplinary research, starting distance learning to enhance qualifications, and the perception that due to online teaching, there is more time for personal development. We employ a set of ordinal regression analyses controlling for age, gender, position (lecturer, researcher, and manager), type of science (formal, natural, social, and applied), and country. The results suggest that Emotional Creativity and its three components predict the motivation of university professors to creatively capitalize on the imposed online teaching in looking for innovative research and personal development under the conditions of COVID-19. Furthermore, our results confirmed the gender and age differences in EC. The differences in EC according to position (lecturer, researcher, and manager) and type of science were not statistically significant. These results compel us to be aware of the importance of the emotional side of creativity to optimize stress-related behavior under the conditions of limited abilities to continue as usual. More space devoted to the manifestation of all the aspects of emotional creativity would improve adaptation to challenging circumstances and even allow one to capitalize on new opportunities. Moreover, we suggest that if personal intrinsic Emotional Creativity is high, the crises, such as the COVID-19 epidemic, may improve adaptation and trigger creative outcomes.

• Publikační typ
• časopisecké články MeSH

Nanotechnology, new fascinating field of science, is bringing many application's options. However, it is necessary to understand their potential environmental risk and toxicity. Zinc selenide quantum dots (ZnSe QDs) are getting valuable due to wide industrial usage, mainly as cadmium free diodes or stabilizing ligand. Thanks to unique properties, they could also open the possibilities of application in the agriculture. Their effects on living organisms, including plants, are still unknown. Therefore, the attention of this work was given to antioxidant response of Arabidopsis thaliana to 100 and 250 μM ZnSe QDs foliar feeding. ZnSe QDs treatment had no statistically significant differences in morphology but led to increased antioxidant response in the leaves at the level of gene expression and production secondary antioxidant metabolites. Concurrently, analysis of growth properties of Agrobacterium tumefaciens was done. 250 μM ZnSe solution inhibited the Agrobacterium tumefaciens viability by 60%. This is the first mention about effect ZnSe QDs on the plants. Although QDs induced oxidative stress, the apply treatment dose of ZnSe QDs did not have significant toxic effect on the plants and even no morphological changes were observed. However, the same amount of ZnSe QD induced an inhibitory effect on Agrobacterium tumefaciens.

• MeSH
• Agrobacterium tumefaciens účinky léků   růst a vývoj   MeSH
• antioxidancia metabolismus   MeSH
• Arabidopsis genetika   růst a vývoj   metabolismus   MeSH
• exprese genu * MeSH
• kvantové tečky toxicita   MeSH
• metabolomika * MeSH
• sloučeniny selenu aplikace a dávkování   toxicita   MeSH
• sloučeniny zinku aplikace a dávkování   toxicita   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

This work has analysed the influence of CdCl2, NiCl2 and CuCl2 on simple phenolic acids, such as the 3PPP (phenylpyruvic, phenylacetic, and 4-hydroxyphenylpyruvic) and 2DR (3,4-dihydroxyphenylacetic and rosmarinic) phenolic acids for the first time and studied their interactions with antioxidant systems and the glutathione-ascorbate cycle in the freshwater green microalga Scenedesmus quadricauda. The compounds investigated are related to both the catabolic and anabolic pathways of l-phenylalanine and l-tyrosine, the main molecules in the biosynthesis of polyphenols. The concentrations of the simple phenolic acids responded significantly to NiCl2, CdCl2 and CuCl2 at 5, 20 and 40 μM concentrations. The 3PPP phenolic acid concentrations after 24 h were always higher in metal-treated cells than in controls, while the 2DR concentrations were significantly lower in the metal-treated cells than the controls. The GSH/GSSG ratio was lower in all experimental groups treated with the selected metals (especially so at 40 μM). Pearson correlation analysis indicated a strong negative correlation between ascorbate and rosmarinic acid content (-0.670; p < 0.05) in NiCl2-treated samples and reduced glutathione and 3,4-dihydroxyphenylacetic content (-0.700; p < 0.05) in CdCl2-treated samples. The GSSG content in samples exposed to CuCl2 was correlated with the concentrations of all of the investigated phenolic acids (4 negative, 1 positive).

• MeSH
• antioxidancia metabolismus   MeSH
• chlorid kademnatý toxicita   MeSH
• fytochelatiny metabolismus   MeSH
• glutathion metabolismus   MeSH
• hydroxybenzoáty metabolismus   MeSH
• kyselina askorbová metabolismus   MeSH
• měď toxicita   MeSH
• mikrořasy účinky léků   růst a vývoj   metabolismus   MeSH
• nikl toxicita   MeSH
• oxidační stres účinky léků   MeSH
• polyfenoly metabolismus   MeSH
• Scenedesmus účinky léků   růst a vývoj   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH

Xestia Hübner, [1818] is a large noctuine genus with an almost worldwide distribution. In the Palaearctic region the genus represented by about 190 described species (Kononenko 2003; Gyulai & Ronkay 2006; Fibiger et al. 2010; Gyulai et al. 2011; Kononenko et al. 2012; Gyulai et al. 2013a; 2013b). The genus subdivided into four described subgenera (Lafontaine et al. 1998, in Lafontaine 1998; Kononenko et al. 2012): Xestia, Megasema Hübner, [1821], Pachnobia Guenée, 1852 (= Schoyenia Aurivillius, 1883; Anomogyna Staudinger, 1871) and Raddea Alphéraky, 1892 (= Estimata I. Kozhanchikov, 1928; Erebophasma Boursin, 1963). Schoyenia and Anomogyna have been synonymised with Pachnobia by Lafontaine et al. (1998, in Lafontaine 1998). On the status of Raddea and synonymy of Estimata and Erebophasma see Lafontaine (1998). The genus is most diverse in the mountains of South Siberia, West China and the Himalayas. The Holarctic taxa of Pachnobia were revised by Mikkola et al. (1983, as Schoyenia), Lafontaine et al. (1987, as Pachnobia), and Lafontaine et al. (1998, in Lafontaine 1998); European taxa of the genus have been reviewed by Fibiger (1992; 1997), and the Nearctic fauna has been revised by Lafontaine (1998). The Asiatic fauna of the genus needs revision.

• MeSH
• anatomické struktury zvířat anatomie a histologie   růst a vývoj   MeSH
• ekosystém MeSH
• můry anatomie a histologie   klasifikace   růst a vývoj   MeSH
• rozšíření zvířat MeSH
• velikost orgánu MeSH
• velikost těla MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Čína MeSH
• Rusko MeSH

• MeSH
• lidé MeSH
• odborný výcvik MeSH
• první pomoc MeSH
• řízená soutěž MeSH
• třídění pacientů MeSH
• urgentní zdravotnické služby metody   organizace a řízení   MeSH
• záchranná práce metody   pracovní síly   využití   MeSH
• Check Tag
• lidé MeSH

• MeSH
• kongresy jako téma MeSH
• urgentní lékařství * MeSH

• MeSH
• algoritmy MeSH
• bolesti na hrudi diagnóza   MeSH
• cévní mozková příhoda diagnóza   komplikace   MeSH
• diferenciální diagnóza MeSH
• dyspnoe diagnóza   MeSH
• lidé MeSH
• otrava terapie   MeSH
• urgentní lékařství MeSH
• urgentní zdravotnické služby MeSH
• Check Tag
• lidé MeSH