SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The grafts contained numerous Purkinje cells but long distance graft-to-host axonal connections to the deep cerebellar nuclei were rarely seen. Relatively few astrocytes were found in the center of the graft. No signs of inflammation or tissue destruction were seen in the area around the grafts. Despite good graft survival and the presence of graft-derived Purkinje cells, the structure of the graft did not seem to promise any significant specific functional effects. We have shown that the graft is available for long-term experiments. Nevertheless, it would be beneficial to search for ways of enhancement of connections between the graft and host.
- MeSH
- mozeček patologie transplantace MeSH
- myši transgenní MeSH
- přežívání štěpu MeSH
- sexuální faktory MeSH
- spinocerebelární ataxie terapie MeSH
- transplantace fetální tkáně * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- buněčná diferenciace účinky léků MeSH
- buněčné linie cytologie MeSH
- embryonální kmenové buňky cytologie metabolismus účinky léků MeSH
- imunohistochemie metody MeSH
- kmenové buňky embryonálního karcinomu cytologie metabolismus účinky léků MeSH
- MAP kinasa-kinasa 2 analýza MeSH
- myši MeSH
- neurony cytologie metabolismus MeSH
- pilotní projekty MeSH
- tretinoin farmakokinetika farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- techniky in vitro MeSH
Dentin představuje mineralizovanou tvrdou zubní tkáň, která tvoří jádro klinické korunky i kořene. Dentinová tkáň je prostoupena množstvím dentinových tubulů, v jejichž luminech jsou umístěny cytoplazmatické výběžky odontoblastů. Pokud je povrch zubu chráněn vrstvou skloviny a cementu, je zubní dřeň izolována od vnějšího prostředí. Je-li vrstva skloviny nebo cementu poškozena, jsou dentin i zubní dřeň vystaveny prostředí ústní dutiny, a tím i průniku bakterií a jejich toxinů. Cílem prezentované experimentální studie bylo zjistit, na základě penetrace organického barviva, možnost průniku cizorodých látek přes stěnu kořene.
Dentin represents a mineralized hard tooth tissue which forms the core of clinical tooth crown and root. The dentin tissue is filled with numerous dentinal tabules whose lumens contain cytoplasmic processes of odontoblasts. If the tooth surface is protected by the layer of enamel and cement, the dental pulp is isolated from external environment. If the layer of enamel or cement is damaged, dentin and dental pulp are exposed to the environment of oral cavity and therefore to penetration of bacteria and their toxins. The objective of the experimental study was to determine, on the basis of penetrating organic dye, the possibility of penetration of foreign compounds across the root wall.
- Publikační typ
- abstrakt z konference MeSH
