The antiyeast activity of the black cumin seed (Nigella sativa) quinones dithymoquinone, thymohydroquinone (THQ), and thymoquinone (TQ) were evaluated in vitro with a broth microdilution method against six dairy spoilage yeast species. Antifungal effects of the quinones were compared with those of preservatives commonly used in milk products (calcium propionate, natamycin, and potassium sorbate) at two pH levels (4.0 and 5.5). THQ and TQ possessed significant antiyeast activity and affected the growth of all strains tested at both pH levels, with MICs ranging from 8 to 128 μg/ml. With the exception of the antibiotic natamycin, the inhibitory effects of all food preservatives against the yeast strains tested in this study were strongly affected by differences in pH, with MICs of ≥16 and ≥512 μg/ml at pH 4.0 and 5.5, respectively. These findings suggest that HQ and TQ are effective antiyeast agents that could be used in the dairy industry as chemical preservatives of natural origin.
- MeSH
- antifungální látky farmakologie MeSH
- chinony farmakologie MeSH
- koncentrace vodíkových iontů MeSH
- konzervace potravin metody MeSH
- kvasinky účinky léků růst a vývoj MeSH
- lidé MeSH
- mléko mikrobiologie MeSH
- Nigella sativa chemie MeSH
- počet mikrobiálních kolonií MeSH
- potravinářské konzervační látky farmakologie MeSH
- spotřebitelská bezpečnost produktů MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Both bifidobacteria and clostridia are part of the natural gut microflora and while clostridia may be responsible for severe intestinal infections, bifidobacteria are probiotic microorganisms belonging to the most important prospective bacteria in the bowel. The antimicrobial activity of biochanin A was tested in vitro against six Bifidobacterium spp., and eight Clostridium spp. using the broth microdilution method. Biochanin A showed an inhibition against all clostridia in the range of minimum inhibitory concentrations (MIC) from 64 microg/mL (for Cl. clostridioforme, strains DSM 933 and I3) to 1,024 microg/mL (for Cl. perfringens, DSM 11778). No bifidobacteria were suppressed at four-fold higher concentration (MICs > 4,096) than MIC of Cl. perfringens. These results indicate selective growth inhibition of biochanin A and its potential use in antimicrobial prevention and/or protection.
- MeSH
- antibakteriální látky farmakologie MeSH
- Bifidobacterium klasifikace účinky léků růst a vývoj MeSH
- Clostridium klasifikace účinky léků růst a vývoj MeSH
- druhová specificita MeSH
- genistein farmakologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- střeva mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
