Oral administration of Lactobacillus spp. as probiotics is gaining importance in the treatment of intestinal inflammations. However, their mechanism of action is unknown. We investigated whether nonspecific binding Lactobacillus casei Shirota (LcS) and mannose-specific Lactobacillus plantarum 299v (Lp) and their spent culture supernatant (SCS) affect Salmonella enteritidis 857 (Se) growth, IL-8 and Hsp70 syntheses. In one set of experiments human enterocyte-like Caco-2 cells were infected with LcS, Lp or Se at 1-500 bacteria per cell for 1 h. In another set, cells were exposed to Se (0-200 per cell, 1 h) after exposure to lactobacilli (LB) (500 per cell, 30 min) or by co-incubation of Se and LB (1 h). The third set of experiments involved exposure of cells for 1 h to SCS or Se (100 per cell) pretreated (1 h) in SCS. The effect of LB SCS on Se growth was evaluated by agar plate diffusion test. IL-8 and Hsp70 were assessed over 2-24 h using ELISA and Western blotting, respectively. Neither LcS nor Lp affected the Se growth and IL-8 production. In addition, they did not induce Hsp70 expression by Caco-2 cells. Instead, their SCS inhibited the Se growth and IL-8 production and induced the expression of Hsp70 by both crypt- and villus-like cells. The beneficial effect of Lactobacillus spp. to the intestinal inflammations might be associated with a decrease in IL-8 levels. This effect could be mediated, at least in part, via a secreted antimicrobial product(s) either directly against the pathogens or indirectly through the synthesis of Hsp70.

• MeSH
• Caco-2 buňky MeSH
• ELISA MeSH
• imunologické faktory biosyntéza   MeSH
• interleukin-8 antagonisté a inhibitory   imunologie   MeSH
• kokultivační techniky MeSH
• kultivační média chemie   MeSH
• Lactobacillus casei metabolismus   MeSH
• Lactobacillus plantarum metabolismus   MeSH
• lidé MeSH
• probiotika MeSH
• proteiny tepelného šoku HSP70 biosyntéza   MeSH
• Salmonella enteritidis patogenita   růst a vývoj   MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH

The intestinal environment accommodates a wide range of contents ranging from harmless beneficial dietary and microbial flora to harmful pathogenic bacteria. This has resulted in the development of highly adapted epithelial cells lining the intestine. This adaptation involves the potential of crypt cells to proliferate and to constantly replace villous cells that are lost due to maturity or death. As a result, the normal intestinal epithelial integrity and functions are maintained. This phenomenon is eminent in intestinal defense whereby the intestinal epithelial cells serve as a physical barrier against luminal agents. The protection against agents in the gut lumen can only be effective if the epithelium is intact. Restitution of the damaged epithelium is therefore crucial in this type of defense.

• MeSH
• antibióza MeSH
• Bacteria patogenita   růst a vývoj   MeSH
• bakteriální infekce mikrobiologie   prevence a kontrola   MeSH
• Caco-2 buňky MeSH
• epitelové buňky imunologie   metabolismus   mikrobiologie   MeSH
• gastrointestinální nemoci mikrobiologie   prevence a kontrola   MeSH
• lidé MeSH
• probiotika MeSH
• proteiny tepelného šoku metabolismus   MeSH
• střeva cytologie   imunologie   mikrobiologie   MeSH
• Check Tag
• lidé MeSH