The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.

• MeSH
• ELISA MeSH
• králíci MeSH
• monoklonální protilátky biosyntéza   MeSH
• nemoci rostlin virologie   MeSH
• Potyvirus imunologie   MeSH
• protilátky virové biosyntéza   MeSH
• rekombinantní proteiny imunologie   MeSH
• Solanum tuberosum virologie   MeSH
• tabák virologie   MeSH
• virové plášťové proteiny imunologie   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH

Vaccine strategies for the treatment of human papillomavirus-induced cervical cancer are based mainly on the human papillomavirus 16 E7 (HPV16 E7) oncoprotein. The immunogenicity of the E7 gene has been enhanced by its fusion to many different genes. Here, we linked a short sequence coding for the E7 peptide (aa 44-60) containing immunodominant epitopes for B and T cells to the 3' end of the gene coding for the whole coat protein (CP) of the poty-virus, potato virus A (PVA), and its deleted form (CPdel) with a short C-terminal deletion of 5 amino acids (LGVKG). CP-E7 and CPdel-E7 fusion proteins, just like CP alone, spontaneously assembled into virus-like particles in both procaryotic and eucaryotic cells. The CP-E7 and CPdel-E7 fusion genes induced slightly stronger E7-specific cytotoxic T-lymphocyte responses than the whole E7 gene, although they were still lower than those elicited by the previously constructed fusion gene, Sig/E7GGG/LAMP-1. The E7- and CP-specific antibody responses were not detected in mice vaccinated with CP-E7 and CPdel-E7 fusion genes. The CP-E7 and CPdel-E7 fusion genes protected mice against the development of tumors induced by TC-1 cells producing the E7 antigen and were also effective in the therapeutic setting, i.e. when the vaccination was performed after tumor cell administration. Their antitumor effect was comparable to those of the whole E7 gene and Sig/E7GGG/LAMP-1 fusion gene. There was no relevant difference between immune responses elicited by CP-E7 and CPdel-E7 DNA vaccination.

• MeSH
• antitumorózní látky farmakologie   MeSH
• biolistika MeSH
• buněčné linie MeSH
• buňky NIH 3T3 MeSH
• časové faktory MeSH
• cytotoxické T-lymfocyty cytologie   MeSH
• DNA vakcíny MeSH
• ELISA MeSH
• financování organizované MeSH
• genetická terapie metody   MeSH
• hmotnostní spektrometrie MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• onkogenní proteiny virové chemie   MeSH
• peptidy chemie   MeSH
• plazmidy metabolismus   MeSH
• Potyvirus genetika   MeSH
• protinádorové vakcíny MeSH
• rekombinantní fúzní proteiny chemie   MeSH
• RNA chemie   MeSH
• sekvence aminokyselin MeSH
• sekvenční homologie aminokyselin MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• technika přenosu genů MeSH
• transmisní elektronová mikroskopie MeSH
• transplantace nádorů MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH