Cieľom práce bolo sledovanie potenciálnych antibiofilmových vlastností biosurfaktantov (BS) izolovaných z kmeňa Bacillus amyloliquefaciens 3/22 voči tvorbe biofilmu indikátorového kmeňa Staphylococcus aureus CCM 4223. V práci bol sledovaný účinok BS 3/22 na rast biofilmu počas spoločnej inkubácie, inhibícia adhézie biofilm tvoriacich buniek a disperzia biofilmu. BS 3/22 inhibovali tvorbu biofilmu, pričom sa jeho tvorba s rastúcou koncentráciou BS signifikantne (p < 0,05; p < 0,01; p < 0,001) znižovala. BS 3/22 preukázali aj antiadhezívnu aktivitu, ktorá korelovala s použitou koncentráciou. Detegovaný bol taktiež disperzný efekt izolovaných BS 3/22 na 24-hodinový biofilm S. aureus CCM 4223. BS 3/22 boli pri disperzii biofilmu účinné aj pri nižších koncentráciách v porovnaní s antiadhezívnou aktivitou a inhibíciou tvorby biofilmu.
The aim of this work was to monitor the potential antibiofilm properties of biosurfactants (BS) isolated from Bacillus amyloliquefaciens 3/22 against biofilm formation of the indicator strain Staphylococcus aureus CCM 4223. In this work, the effect of BS 3/22 on biofilm growth during co-incubation, inhibition of biofilm-forming cell adhesion and biofilm dispersion was studied. BS 3/22 inhibited biofilm formation, with its formation decreasing significantly (p < 0.05; p < 0.01; p < 0.001) with increasing BS 3/22 concentration. BS 3/22 also showed antiadhesive activity, which correlated with the concentration used. The dispersing effect of isolated BS 3/22 on a 24-hour biofilm was also detected. BS 3/22 were effective in biofilm dispersion even at lower concentrations compared to antiadhesive activity and inhibition of biofilm formation.
- Klíčová slova
- biosurfaktanty,
- MeSH
- Bacillus amyloliquefaciens MeSH
- biofilmy * účinky léků MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
[Probiotic products as biological active substances]
Probiotics and their products, due to their beneficial biological properties, participate in optimum functioning of organ systems and form a natural barrier against the overgrowth of undesirable microorganisms. Probiotic bacteria produce biologically active substances, such as bacteriocins, biosurfactants, vitamin K2, and exopolysaccharides. Inhibition of growth and metabolic activity of technologically and medically undesirable microflora, antimicrobial and antiadhesive properties or antibiofilm activity of these substances represent a wide range of their application in medicine, industry and food industry.
Rezistencia patogénnych baktérií je v dnešnej dobe jedným z hlavných medicínskych problémov. Väčšina mikrobiálnych infekcií má základ v tvorbe biofilmov, ktoré sú významným rezervoárom patogénov. Táto práca sa zaoberá antibiofilmovou a antimikrobiálnou aktivitou biosurfaktantov z črevných laktobacilov a baktérií izolovaných zo vzoriek mora. Biosurfaktanty (BS) izolované z kmeňov L. fermentum 2I3, L. fermentum B2/6, L. reuteri SL16, L. reuteri B6/1, S. luteola 3/22, Brevibacillus sp. 4/9, Brevibacillus sp. 2/30 a B. amyloliquefaciens 1/6K signifikantne (p < 0,001) inhibovali tvorbu biofilmu u S. aureus CCM 3953 a P. mirabilis CCM 7188, pričom výraznejšia inhibícia bola detegovaná v prípade BS morských baktérií oproti laktobacilovým BS. Výsledky naznačujú, že mechanizmus antibiofilmového efektu laktobacilových BS voči obidvom referenčným kmeňom je rovnaký a nie je výsledkom ich antimikrobiálneho pôsobenia. Naproti tomu, mechanizmus antibiofilmového efektu BS získaných z morských baktérií je pravdepodobne závislý od vlastností použitého referenčného kmeňa.
Resistance of pathogenic bacteria is currently one of the major medical problems. Most microbial infections are based on the formation of biofilms, which are a significant reservoir of pathogens. The aim of this study is to determine the antibiofilm and antimicrobial activity of biosurfactants isolated from intestinal lactobacilli and marine bacteria. Biosurfactants (BS) isolated from the strains L. fermentum 2I3, L. fermentum B2/6, L. reuteri SL16, L. reuteri B6/1, S. luteola 3/22, Brevibacillus sp. 4/9, Brevibacillus sp. 2/30 and B. amyloliquefaciens 1/6K significantly (p < 0.001) inhibited the biofilm formation of S. aureus CCM 3953 and P. mirabilis CCM 7188, with higher inhibition detected in BS of marine bacteria when compared to BS isolated from lactobacilli. The results suggest that the mechanism of the antibiofilm effect of BS isolated from lactobacilli against both the reference strains is the same and it is not the result of their antimicrobial action. In contrast, the mechanism of the antibiotic effect of BS isolated from marine bacteria probably depends on the properties of the indicator strain.
- Klíčová slova
- biosurfaktanty,
- MeSH
- antibakteriální látky * farmakologie chemie metabolismus MeSH
- biofilmy * účinky léků MeSH
- lidé MeSH
- mořská voda mikrobiologie MeSH
- povrchově aktivní látky farmakologie metabolismus MeSH
- výzkum MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Probiotics are beneficial microorganisms, defined as "live microorganisms which, when administered in a reasonable amount, provide health benefits for the host". Not only the types of bacteria themselves, but also various other factors such as the ability to produce exopolysaccharides, biofilms, etc. significantly affect the positive effect of probiotics. Probiotic bacteria are extremely efficient in the production of biologically active substances – exopolysaccharides. These substances are important for their multilateral effects on the human organism. Significant properties of the exopolysaccharides produced by the lactic acid bacteria include anti-biofilm activity, prebiotic properties and other impacts of these substances on both beneficial and pathogenic bacteria of the digestive tract.
- MeSH
- biofilmy MeSH
- polysacharidy MeSH
- probiotika * MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- dieta MeSH
- gnotobiologické modely fyziologie MeSH
- mikroklky fyziologie patologie MeSH
- prasata růst a vývoj MeSH
- tenké střevo růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- Bacteria metabolismus MeSH
- Enterococcus faecium metabolismus MeSH
- Escherichia coli metabolismus MeSH
- extracelulární matrix - proteiny metabolismus MeSH
- finanční podpora výzkumu jako téma MeSH
- Lactobacillus metabolismus MeSH
- muciny metabolismus MeSH
- probiotika metabolismus MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH