Cieľom práce bolo sledovanie potenciálnych antibiofilmových vlastností biosurfaktantov (BS) izolovaných z kmeňa Bacillus amyloliquefaciens 3/22 voči tvorbe biofilmu indikátorového kmeňa Staphylococcus aureus CCM 4223. V práci bol sledovaný účinok BS 3/22 na rast biofilmu počas spoločnej inkubácie, inhibícia adhézie biofilm tvoriacich buniek a disperzia biofilmu. BS 3/22 inhibovali tvorbu biofilmu, pričom sa jeho tvorba s rastúcou koncentráciou BS signifikantne (p < 0,05; p < 0,01; p < 0,001) znižovala. BS 3/22 preukázali aj antiadhezívnu aktivitu, ktorá korelovala s použitou koncentráciou. Detegovaný bol taktiež disperzný efekt izolovaných BS 3/22 na 24-hodinový biofilm S. aureus CCM 4223. BS 3/22 boli pri disperzii biofilmu účinné aj pri nižších koncentráciách v porovnaní s antiadhezívnou aktivitou a inhibíciou tvorby biofilmu.
The aim of this work was to monitor the potential antibiofilm properties of biosurfactants (BS) isolated from Bacillus amyloliquefaciens 3/22 against biofilm formation of the indicator strain Staphylococcus aureus CCM 4223. In this work, the effect of BS 3/22 on biofilm growth during co-incubation, inhibition of biofilm-forming cell adhesion and biofilm dispersion was studied. BS 3/22 inhibited biofilm formation, with its formation decreasing significantly (p < 0.05; p < 0.01; p < 0.001) with increasing BS 3/22 concentration. BS 3/22 also showed antiadhesive activity, which correlated with the concentration used. The dispersing effect of isolated BS 3/22 on a 24-hour biofilm was also detected. BS 3/22 were effective in biofilm dispersion even at lower concentrations compared to antiadhesive activity and inhibition of biofilm formation.
- Klíčová slova
- biosurfaktanty,
- MeSH
- Bacillus amyloliquefaciens MeSH
- biofilmy * účinky léků MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
The aim of this study was to investigate the use of a standardized animal model subjected to antibiotic treatment, and the effects of this treatment on the course of dextran sodium sulphate (DSS)-induced colitis in mice. By decontamination with selective antibiotics and observation of pathogenesis of ulcerative colitis (UC) induced chemically by exposure of mice to various concentrations of DSS, we obtained an optimum animal PGF model of acute UC manifested by mucin depletion, epithelial degeneration and necrosis, leading to the disappearance of epithelial cells, infiltration of lamina propria and submucosa with neutrophils, cryptitis, and accompanied by decreased viability of intestinal microbiota, loss of body weight, dehydration, moderate rectal bleeding, and a decrease in the selected markers of cellular proliferation and apoptosis. The obtained PGF model did not exhibit changes that could contribute to inflammation by means of alteration of the metabolic status and the induced dysbiosis did not serve as a bearer of pathogenic microorganisms participating in development of ulcerative colitis. The inflammatory process was induced particularly by exposure to DSS and its toxic action on compactness and integrity of mucosal barrier in the large intestine. This offers new possibilities of the use of this animal model in studies with or without participation of pathogenic microbiota in IBD pathogenesis.
- MeSH
- antibakteriální látky farmakologie MeSH
- apoptóza fyziologie MeSH
- epitelové buňky patologie MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- proliferace buněk fyziologie MeSH
- síran dextranu farmakologie MeSH
- střevní mikroflóra účinky léků fyziologie MeSH
- střevní sliznice mikrobiologie patologie MeSH
- ulcerózní kolitida chemicky indukované farmakoterapie patologie MeSH
- zánět farmakoterapie patologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
[Probiotic products as biological active substances]
Probiotics and their products, due to their beneficial biological properties, participate in optimum functioning of organ systems and form a natural barrier against the overgrowth of undesirable microorganisms. Probiotic bacteria produce biologically active substances, such as bacteriocins, biosurfactants, vitamin K2, and exopolysaccharides. Inhibition of growth and metabolic activity of technologically and medically undesirable microflora, antimicrobial and antiadhesive properties or antibiofilm activity of these substances represent a wide range of their application in medicine, industry and food industry.
Rezistencia patogénnych baktérií je v dnešnej dobe jedným z hlavných medicínskych problémov. Väčšina mikrobiálnych infekcií má základ v tvorbe biofilmov, ktoré sú významným rezervoárom patogénov. Táto práca sa zaoberá antibiofilmovou a antimikrobiálnou aktivitou biosurfaktantov z črevných laktobacilov a baktérií izolovaných zo vzoriek mora. Biosurfaktanty (BS) izolované z kmeňov L. fermentum 2I3, L. fermentum B2/6, L. reuteri SL16, L. reuteri B6/1, S. luteola 3/22, Brevibacillus sp. 4/9, Brevibacillus sp. 2/30 a B. amyloliquefaciens 1/6K signifikantne (p < 0,001) inhibovali tvorbu biofilmu u S. aureus CCM 3953 a P. mirabilis CCM 7188, pričom výraznejšia inhibícia bola detegovaná v prípade BS morských baktérií oproti laktobacilovým BS. Výsledky naznačujú, že mechanizmus antibiofilmového efektu laktobacilových BS voči obidvom referenčným kmeňom je rovnaký a nie je výsledkom ich antimikrobiálneho pôsobenia. Naproti tomu, mechanizmus antibiofilmového efektu BS získaných z morských baktérií je pravdepodobne závislý od vlastností použitého referenčného kmeňa.
Resistance of pathogenic bacteria is currently one of the major medical problems. Most microbial infections are based on the formation of biofilms, which are a significant reservoir of pathogens. The aim of this study is to determine the antibiofilm and antimicrobial activity of biosurfactants isolated from intestinal lactobacilli and marine bacteria. Biosurfactants (BS) isolated from the strains L. fermentum 2I3, L. fermentum B2/6, L. reuteri SL16, L. reuteri B6/1, S. luteola 3/22, Brevibacillus sp. 4/9, Brevibacillus sp. 2/30 and B. amyloliquefaciens 1/6K significantly (p < 0.001) inhibited the biofilm formation of S. aureus CCM 3953 and P. mirabilis CCM 7188, with higher inhibition detected in BS of marine bacteria when compared to BS isolated from lactobacilli. The results suggest that the mechanism of the antibiofilm effect of BS isolated from lactobacilli against both the reference strains is the same and it is not the result of their antimicrobial action. In contrast, the mechanism of the antibiotic effect of BS isolated from marine bacteria probably depends on the properties of the indicator strain.
- Klíčová slova
- biosurfaktanty,
- MeSH
- antibakteriální látky * farmakologie chemie metabolismus MeSH
- biofilmy * účinky léků MeSH
- lidé MeSH
- mořská voda mikrobiologie MeSH
- povrchově aktivní látky farmakologie metabolismus MeSH
- výzkum MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Probiotics are beneficial microorganisms, defined as "live microorganisms which, when administered in a reasonable amount, provide health benefits for the host". Not only the types of bacteria themselves, but also various other factors such as the ability to produce exopolysaccharides, biofilms, etc. significantly affect the positive effect of probiotics. Probiotic bacteria are extremely efficient in the production of biologically active substances – exopolysaccharides. These substances are important for their multilateral effects on the human organism. Significant properties of the exopolysaccharides produced by the lactic acid bacteria include anti-biofilm activity, prebiotic properties and other impacts of these substances on both beneficial and pathogenic bacteria of the digestive tract.
- MeSH
- biofilmy MeSH
- polysacharidy MeSH
- probiotika * MeSH
- Publikační typ
- práce podpořená grantem MeSH
Kocuria spp. are widely distributed in nature. They are Gram-positive, coagulase-negative, coccoid bacteria belonging to the family Micrococcaceae, suborder Micrococcineae, order Actinomycetales, class Actinobacteria. In general, limited knowledge exists concerning the properties associated with the representants of the genus Kocuria, Kocuria kristinae as well. Following our previous results, K. kristinae Kk2014 Biocenol(™) (CCM 8628) was isolated from vagina of a healthy cow. Its taxonomical allottation was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) identification system and phenotypic characteristics. Kk2014 strain showed strong adherence capability to the vaginal mucus, produced organic acids which can play a role in prevention of unsuitable contamination, and showed in vitro antagonistic/antimicrobial activity against strains Arcanobacterium pyogenes CCM 5753, Fusobacterium necrophorum CCM 5982, Streptococcus equi subsp. zooepidemicus CCM 7316, and Gardnerella vaginalis CCM 6221. Antimicrobial activity ranged from 100 to 200 AU/mL, up to 32 mm in size, respectively.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální adheze MeSH
- fenotyp MeSH
- grampozitivní bakteriální infekce veterinární MeSH
- Micrococcaceae klasifikace účinky léků izolace a purifikace MeSH
- mikrobiální testy citlivosti MeSH
- skot MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- vagina mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of the study was to investigate the influence of flaxseed and lactobacilli supplementation to the diet of piglets during the time period between 10 days before and 21 days after weaning. The morphometry of the jejunal mucosa and proliferative ratio of both epithelial and lamina propria cells were compared with those found in a group of piglets fed with the usual diet added with sunflower oil during the same time period. The addition of flaxseed oil to the diet significantly increased the crypt depth in comparison with both groups supplemented with sunflower (P < 0.05 and 0.001 respectively) on the weaning day. Moreover, the flaxseed addition caused a significant decrease in villus height (P < 0.01) and crypt depth (P < 0.01) 21 days postweaning in comparison with the sunflower group. The proliferative ratio of the epithelial cells in the sunflower group on the weaning day was significantly higher than in both flaxseed groups (P < 0.01). Paradoxically, significantly higher proliferative activity in the mucosal connective tissue in the group with flaxseed supplementation in comparison with the sunflower group was observed on the day of weaning, as well as 3 days later (P < 0.05 both). A combination of flaxseed with lactobacilli showed significantly lower proliferative activity in the connective tissue cells from weaning up to 7 days after weaning (P < 0.05 all) in comparison with the flaxseed group.
- MeSH
- časové faktory MeSH
- fyziologie výživy zvířat MeSH
- jejunum účinky léků růst a vývoj mikrobiologie MeSH
- krmivo pro zvířata * MeSH
- Lactobacillus fyziologie MeSH
- lněný olej aplikace a dávkování MeSH
- odstavení MeSH
- prasata MeSH
- probiotika * MeSH
- proliferace buněk účinky léků MeSH
- střevní sliznice účinky léků růst a vývoj mikrobiologie MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
A histological study was designed to determine the influence of flaxseed and/or lactobacilli inclusion in the diet of piglets from 10 days before to 21 days after weaning. The selected inflammatory cell population incidence in the piglet jejunal mucosa was investigated. Significantly higher numbers of myeloperoxidase-positive (P<0.01) and CD163-positive (P<0.001) cells in the jejunal mucosa were recorded on the weaning day and for 7 days after (P<0.001 and P<0.01, respectively) in the flaxseed group compared with the basal diet. The number of intraepithelial lymphocytes was also significantly increased until 3 days after weaning (P<0.001). A prolonged significant increase in the myeloperoxidase-positive cells and intraepithelial lymphocyte numbers in the flaxseed+lactobacilli group was detected. In contrast, the number of CD163-positive cells in the flaxseed+lactobacilli group was significantly lower on the day of weaning (P<0.05) and 3 days after (P<0.01). The same effect was observed in the group with lactobacilli alone during the first 3 days after weaning (P<0.05 and P<0.01, respectively) and these findings indicate down-regulation of CD163 expression in the jejunal mucosa by lactobacilli. The presence of lactobacilli in the diet had a stimulatory effect on goblet cell quantity in the epithelium (P<0.001) and a distinct 50% reduction in the flaxseed group (P<0.01) compared with the basal diet was observed on the weaning day. A significant increase in myeloperoxidase-positive cell number in the jejunal mucosa in the flaxseed+lactobacilli group was the only significant difference (P<0.05 and P<0.01, respectively) found 21 days after weaning in comparison with all the other groups, indicating the pro-inflammatory effect of this feed additive combination. We conclude that dietary supplementation with flaxseed and lactobacilli on the cells of local innate immunity response in the jejunal mucosa in piglets after weaning might be linked with significant anti-inflammatory effects in the jejunal mucosa.
- MeSH
- jejunum mikrobiologie MeSH
- Lactobacillus * MeSH
- len * MeSH
- odstavení * MeSH
- potravní doplňky mikrobiologie MeSH
- prasata MeSH
- střevní sliznice mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
