- Klíčová slova
- metoda Sapere, projekt Jíme všemi smysly,
- MeSH
- dítě MeSH
- lidé MeSH
- potraviny a nápoje * MeSH
- smyslové orgány * fyziologie MeSH
- stravovací zvyklosti MeSH
- výchova a vzdělávání metody MeSH
- zpětná vazba MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
In both mitosis and meiosis, metaphase to anaphase transition requires the activity of a ubiquitin ligase known as anaphase promoting complex/cyclosome (APC/C). The activation of APC/C in metaphase is under the control of the checkpoint mechanism, called the spindle assembly checkpoint (SAC), which monitors the correct attachment of all kinetochores to the spindle. It has been shown previously in somatic cells that exposure to a small molecule inhibitor, prodrug tosyl-l-arginine methyl ester (proTAME), resulted in cell cycle arrest in metaphase, with low APC/C activity. Interestingly, some reports have also suggested that the activity of SAC is required for this arrest. We focused on the characterization of proTAME inhibition of cell cycle progression in mammalian oocytes and embryos. Our results show that mammalian oocytes and early cleavage embryos show dose-dependent metaphase arrest after exposure to proTAME. However, in comparison to the somatic cells, we show here that the proTAME-induced arrest in these cells does not require SAC activity. Our results revealed important differences between mammalian oocytes and early embryos and somatic cells in their requirements of SAC for APC/C inhibition. In comparison to the somatic cells, oocytes and embryos show much higher frequency of aneuploidy. Our results are therefore important for understanding chromosome segregation control mechanisms, which might contribute to the premature termination of development or severe developmental and mental disorders of newborns.
- MeSH
- anafázi podporující komplex metabolismus MeSH
- embryo savčí účinky léků metabolismus MeSH
- embryonální vývoj účinky léků MeSH
- kontrolní body M fáze buněčného cyklu * MeSH
- myši MeSH
- oocyty účinky léků růst a vývoj metabolismus MeSH
- prekurzory léčiv MeSH
- skot MeSH
- tosylargininmethylester aplikace a dávkování farmakologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Early embryonic development is characterized by a plethora of very complex and simultaneously operating processes, which are constantly changing cellular morphology and behaviour. After fertilization, blastomeres of the newly created embryo undergo global epigenetic changes and simultaneously initiate transcription from the zygotic genome and differentiation forming separate cell lineages. Some of these mechanisms were extensively studied during the last several decades and valuable insight was gained into how these processes are regulated at the molecular level. We have, however, a still very limited understanding of how multiple events are coordinated during rapid development of an early mammalian embryo. In this review, we discuss some aspects of early embryonic development in mammals, namely the fidelity of chromosome segregation and occurrence of aneuploidy, as well as the clinical applications of cell cycle monitoring in human embryos.
- MeSH
- aneuploidie MeSH
- aparát dělícího vřeténka metabolismus MeSH
- blastomery metabolismus MeSH
- buněčný cyklus genetika MeSH
- embryo savčí cytologie metabolismus MeSH
- embryonální vývoj genetika MeSH
- lidé MeSH
- segregace chromozomů genetika MeSH
- těhotenství MeSH
- zvířata MeSH
- zygota cytologie metabolismus MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Příspěvek seznamuje s nejčastěji praktikovanými aktivitami využívanými při skupinové canisterapii s dětmi. V úvodu jsou přiblíženy cílové skupiny dětí, u nichž členové Výcvikového canisterapeutického sdružení HAFÍK provádějí skupinovou formu canisterapie a plní cíle stanovované u dětí. V hlavní časti příspěvku jsou představeny již konkrétní canisterapeutické a kynologické aktivity praktikované u dětí.
The contribution familiarizes the reader with most frequently practised activities employed in the group canistherapy in children. In introduction, target groups of children are described, in which members of the Training Canistherapeutic Association HAFÍK perform a group form of canistherapy and follow targets established in children. In the main part of the contribution, particular canistherapeutic and cynological activities practised in children are presented.
