The aim of the study was to compare 4 modes of ischaemic cardioprotection using continuous prograde autologous blood perfusion of the coronary artery in two hypothermic modes (group A, B) or conventional protection by cooled Hartmann solution (group C) or cooled saline (group D) without perfusion of the graft. Male Wistar rats (n = 24) were divided into four groups (A–D). In groups A (22–25 °C) and B (4–8 °C), blood perfusion rate was 10 ml/h and the graft was placed in a water bath. Groups C, D were initially rinsed with cold (4–8 °C) Hartmann solution (C) and cold saline solution (D), next the graft was placed in a water bath of cold (4–8 °C) Hartmann solution (C) or saline solution (D). The observed time was 30 min after the implemented perfusion (A, B) or initial rinsing (C, D). At 30 min, hearts of all the groups were perfused for 10 min with prograde-autologous arterialized blood at room temperature. At perfusion minute 10, blood was collected for biochemical analysis (sample 1). Sample 2 involved blood from a portable syringe infusion pumps (in parallel with sample 1). Pairwise test differences between samples 1 and 2 were significant in all the groups as regards creatine kinase and lactate dehydrogenase values, sampling 1 values being always higher, while cardiac troponin I concentrations were non-significant in the same comparison. The heart rate during the final perfusion was identical in all the groups. Our study has demonstrated that all observed cardioprotection modes are useful for experimental heart grafting.
- MeSH
- hypotermie terapie MeSH
- krysa rodu rattus MeSH
- krystaloidní roztoky MeSH
- obnova funkce MeSH
- přežívání štěpu MeSH
- studená ischemie * MeSH
- Check Tag
- krysa rodu rattus MeSH
- Publikační typ
- práce podpořená grantem MeSH
OBJECTIVES: The aim of this study was to evaluate changes in hematology and coagulation in rabbits with right-ventricle pacing without medication. ANIMALS AND METHODS: Blood was collected from ten non-anesthetized male rabbits from the jugular vein before and one month after pacemaker placement. Total erythrocyte, leukocyte and platelet count, hemoglobin, hematocrit and differential leukocyte count were done on automatic veterinary flow cytometry hematologic analyzer. Prothrombin time, activated partial thromboplastin time, fibrinogen level, D-dimers and kaolin-activated thromboelastography was measured from citrated blood. RESULTS: We found an increase in red blood cell mass and decrease in platelet count, while coagulation tests did not diff er between samplings. CONCLUSION: Right-ventricle pacing seems to have no influence on hemostasis in rabbits.
- MeSH
- fibrin-fibrinogen - produkty degradace MeSH
- fibrinogen MeSH
- hematokrit * MeSH
- hemoglobiny * MeSH
- hemokoagulace MeSH
- hemostáza * MeSH
- kardiostimulace umělá * MeSH
- kardiostimulátor * MeSH
- králíci MeSH
- krevní obraz MeSH
- parciální tromboplastinový čas MeSH
- počet erytrocytů MeSH
- počet leukocytů MeSH
- počet trombocytů MeSH
- protrombinový čas MeSH
- průtoková cytometrie MeSH
- srdeční komory * MeSH
- tromboelastografie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- mužské pohlaví MeSH
- zvířata MeSH
