The activity of antagonistic substances produced by Pseudomonas aeruginosa and Lactobacillus acidophilus against the planktonic and sessile populations of Staphylococcus aureus strains was demonstrated. The strongest effects were caused by probiotic L. acidophilus strain - bacteriocin-like inhibitory substances (BLIS) positive. However, the S. aureus A3 growth, adhesion and biofilm formation was also limited by cell-free supernatant of L. acidophilus H-1 (BLIS negative). Moreover, competitive direct interactions were observed between staphylococci and the above bacteria, which influenced the formation of dualspecies aggregates on the surface.

• MeSH
• antibakteriální látky farmakologie   metabolismus   MeSH
• antibióza MeSH
• bakteriální adheze účinky léků   MeSH
• bakteriociny farmakologie   metabolismus   MeSH
• biofilmy účinky léků   MeSH
• kultivační média speciální farmakologie   MeSH
• Lactobacillus acidophilus fyziologie   metabolismus   růst a vývoj   MeSH
• lidé MeSH
• plankton růst a vývoj   MeSH
• probiotika MeSH
• Pseudomonas aeruginosa fyziologie   metabolismus   růst a vývoj   MeSH
• Staphylococcus aureus fyziologie   růst a vývoj   účinky léků   MeSH
• Check Tag
• lidé MeSH

Analysis of actA gene sequence polymorphism has been shown to be an effective and relatively inexpensive method for subtyping Listeria monocytogenes isolates, allowing the division of the population of this species into two deeply separate lineages. This sequence-based method as well as PCR-mediated fingerprinting were applied here for the differentiation of 49 isolates of food and clinical origin. Correlation between these two typing approaches was high. Both methods divided the isolates into two lineages, designated I (33 isolates) and II (16 isolates). All the 33 lineage I isolates were assigned to the same, or closely related, six clusters by both typing methods. For the lineage II isolates, PCR fingerprinting was found to be more discriminatory. The isolates were characterized by cell invasion assay. All highly invasive isolates were assigned to lineage I, which constituted a heterogeneous group also containing low-invasive isolates. High-invasive isolates were not found in the genetically determined lineage II. A particular actA cluster, designated Ha, contained all the isolates showing the lowest invasiveness. A common trait of the isolates belonging to this cluster was the presence of a threonine-441 of the deduced ActA sequence instead of the alanine-441 present in the remaining isolates. Thirteen human isolates were classified to lineage I and five to lineage II. A PCR-based method can therefore differentiate L. monocytogenes isolates in accordance with the current phylogenetic model of the evolution of this species.

• MeSH
• bakteriální proteiny genetika   MeSH
• cytologické techniky MeSH
• DNA fingerprinting metody   MeSH
• fylogeneze MeSH
• lidé MeSH
• Listeria monocytogenes genetika   izolace a purifikace   klasifikace   MeSH
• listeriové infekce mikrobiologie   MeSH
• membránové proteiny genetika   MeSH
• nádorové buněčné linie MeSH
• polymerázová řetězová reakce MeSH
• potravinářská mikrobiologie MeSH
• techniky typizace bakterií metody   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH

• MeSH
• cystická fibróza imunologie   mikrobiologie   MeSH
• DNA genetika   imunologie   izolace a purifikace   MeSH
• enterotoxiny analýza   chemická syntéza   imunologie   MeSH
• fenotyp MeSH
• genotyp MeSH
• lidé MeSH
• polymerázová řetězová reakce metody   využití   MeSH
• Staphylococcus aureus imunologie   izolace a purifikace   patogenita   MeSH
• superantigeny analýza   genetika   imunologie   MeSH
• Check Tag
• lidé MeSH