- Klíčová slova
- fluorescenční imunohistochemie,
- MeSH
- autoimunitní nemoci * patologie MeSH
- biopsie MeSH
- fluorescenční protilátková technika nepřímá MeSH
- fluorescenční protilátková technika přímá * metody přístrojové vybavení MeSH
- fluorescenční protilátková technika MeSH
- imunohistochemie MeSH
- kožní nemoci * patologie MeSH
- kryoprezervace MeSH
- lidé MeSH
- odběr tkání a orgánů MeSH
- Check Tag
- lidé MeSH
Eight C-6-geranylflavonoids ( 1- 8) were isolated from an ethanol extract of Paulownia tomentosa fruits. Tomentodiplacone ( 1), 3'- O-methyl-5'-hydroxydiplacone ( 2), 3'- O-methyl-5'- O-methyldiplacone ( 3), and tomentodiplacone B ( 4) were obtained as new compounds, and 3'- O-methyldiplacol ( 5) was isolated for the first time from a natural source. The structures of these new compounds were determined by using mass spectrometry (including HRMS) and 1D and 2D NMR spectroscopy, and the absolute configurations of the compounds were determined by circular dichroism. The antibacterial activities of 1- 8 against seven bacteria and yeast were evaluated.
- MeSH
- antibakteriální látky farmakologie chemie izolace a purifikace MeSH
- Bacteria účinky léků MeSH
- Candida albicans účinky léků MeSH
- cirkulární dichroismus MeSH
- financování organizované MeSH
- flavonoidy farmakologie chemie izolace a purifikace MeSH
- léčivé rostliny chemie MeSH
- mikrobiální testy citlivosti MeSH
- molekulární struktura MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- ovoce chemie MeSH
- Scrophulariaceae chemie MeSH
- Geografické názvy
- Česká republika MeSH
Paulownia tomentosa is a large indecidous tree planted mostly for its fast growing wood and decorative purposes. The tree is also used in traditional Chinese medicine. As a part of our study of natural polyphenols, the fruits of Paulownia tomentosa were extracted by EtOH and than subjected to liquid/liquid extraction. Fractions were analysed by TLC and HPLC to determine presence of phenolic substances. We identified and quantified acteoside (1) and isoacteoside (2) in the EtOAc and n-BuOH extracts; mimulone (3) and diplacone (4) in the MeOH extract. To determine the antiradical activity of extracts we used the anti DPPH and peroxynitrite assays. The activity was expressed as Trolox C equivalents, IC50 for DPPH scavenging and a time dependency course was established. The polyphenols content was determined; results were expressed as gallic acid equivalents. Using these methods we found the fractions of the n-BuOH, EtOAc and MeOH extracts that display antiradical activity, which could be exploited as potential pharmaceuticals.
- MeSH
- fenoly farmakologie izolace a purifikace MeSH
- financování organizované MeSH
- flavonoidy farmakologie izolace a purifikace MeSH
- léčivé rostliny MeSH
- léky rostlinné čínské farmakologie izolace a purifikace MeSH
- ovoce chemie MeSH
- rozpouštědla MeSH
- scavengery volných radikálů farmakologie izolace a purifikace MeSH
- stromy MeSH
