BACKGROUND: The aim of our experiments was to investigate the anti-inflammatory properties of casticin and chrysosplenol D, two flavonoids present in Artemisia annua L. METHODS: Topical inflammation was induced in ICR mice using croton oil. Mice were then treated with casticin or chrysosplenol D. Cutaneous histological changes and edema were assessed. ICR mice were intragastrically administrated with casticin or chrysosplenol D followed by intraperitoneal injection of lipopolysaccharide (LPS). Mouse Raw264.7 macrophage cells were incubated with casticin or chrysosplenol D. Intracellular phosphorylation was detected, and migration was assessed by trans-well assay. HT-29/NFκB-luc cells were incubated with casticin or chrysosplenol D in the presence or absence of LPS, and NF-κB activation was quantified. RESULTS: In mice, administration of casticin (0.5, 1 and 1.5μmol/cm(2)) and chrysosplenol D (1 and 1.5μmol/cm(2)) inhibited croton oil-induced ear edema (casticin: 29.39-64.95%; chrysosplenol D: 37.76-65.89%, all P<0.05) in a manner similar to indomethacin (0.5, 1 and 1.5μmol/cm(2); 55.63-84.58%). Casticin (0.07, 0.13 and 0.27mmol/kg) and chrysosplenol D (0.07, 0.14 and 0.28mmol/kg) protected against LPS-induced systemic inflammatory response syndrome (SIRS) in mice (all P<0.05), in a manner similar to dexamethasone (0.03mmol/kg). Casticin and chrysosplenol D suppressed LPS-induced release of IL-1 beta, IL-6 and MCP-1, inhibited cell migration, and reduced LPS-induced IκB and c-JUN phosphorylation in Raw264.7 cells. JNK inhibitor SP600125 blocked the inhibitory effect of chrysosplenol D on cytokine release. CONCLUSIONS: The flavonoids casticin and chrysosplenol D from A. annua L. inhibited inflammation in vitro and in vivo.

• MeSH
• Artemisia annua * MeSH
• buňky HT-29 MeSH
• dermatitida farmakoterapie   patologie   MeSH
• edém farmakoterapie   patologie   MeSH
• endoteliální buňky pupečníkové žíly (lidské) MeSH
• flavonoidy izolace a purifikace   terapeutické užití   MeSH
• flavony izolace a purifikace   terapeutické užití   MeSH
• léky rostlinné čínské izolace a purifikace   terapeutické užití   MeSH
• lidé MeSH
• myši inbrední ICR MeSH
• myši MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zánět farmakoterapie   patologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Huperzine A, a mixture of compounds suitable for pharmaceutical purposes, has been used as an active substance in traditional Chinese medicine. The compounds mainly rank among lycopodin alkaloids from the plant Huperzia serrata and other plants from the families Huperziaceae and Lycopodiaceae and the Selaginella genus. In these families huperzine A prevails. The compounds are suitable for treatment of the Alzheimer disease (AD) by inhibition of acetylcholinesterase and antagonizing NMDA N-methyl-D-aspartate receptors. Currently clinical tests of huperzine A in the AD treatment are performed. The aim of the present review is to summarize basic facts about huperzine A compounds and discuss their suitability for the therapy.

• MeSH
• acetylcholinesterasa MeSH
• Alzheimerova nemoc * farmakoterapie   patofyziologie   MeSH
• amyloidní plaky MeSH
• aplikace orální MeSH
• cholinesterasové inhibitory * MeSH
• Haplorrhini MeSH
• Huperzia * MeSH
• klinické zkoušky, fáze I jako téma MeSH
• léky rostlinné čínské * aplikace a dávkování   farmakologie   izolace a purifikace   terapeutické užití   MeSH
• lidé MeSH
• neurodegenerativní nemoci epidemiologie   farmakoterapie   MeSH
• preklinické hodnocení léčiv * metody   MeSH
• receptory N-methyl-D-aspartátu antagonisté a inhibitory   MeSH
• senioři MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• Publikační typ
• práce podpořená grantem MeSH

Paulownia tomentosa is a large indecidous tree planted mostly for its fast growing wood and decorative purposes. The tree is also used in traditional Chinese medicine. As a part of our study of natural polyphenols, the fruits of Paulownia tomentosa were extracted by EtOH and than subjected to liquid/liquid extraction. Fractions were analysed by TLC and HPLC to determine presence of phenolic substances. We identified and quantified acteoside (1) and isoacteoside (2) in the EtOAc and n-BuOH extracts; mimulone (3) and diplacone (4) in the MeOH extract. To determine the antiradical activity of extracts we used the anti DPPH and peroxynitrite assays. The activity was expressed as Trolox C equivalents, IC50 for DPPH scavenging and a time dependency course was established. The polyphenols content was determined; results were expressed as gallic acid equivalents. Using these methods we found the fractions of the n-BuOH, EtOAc and MeOH extracts that display antiradical activity, which could be exploited as potential pharmaceuticals.

• MeSH
• fenoly farmakologie   izolace a purifikace   MeSH
• financování organizované MeSH
• flavonoidy farmakologie   izolace a purifikace   MeSH
• léčivé rostliny MeSH
• léky rostlinné čínské farmakologie   izolace a purifikace   MeSH
• ovoce chemie   MeSH
• rozpouštědla MeSH
• scavengery volných radikálů farmakologie   izolace a purifikace   MeSH
• stromy MeSH