Microorganisms that cause chronic infections exist predominantly as surface-attached stable communities known as biofilms. Microbial cells in biofilms are highly resistant to conventional antibiotics and other forms of antimicrobial treatment; therefore, modern medicine tries to develop new drugs that exhibit anti-biofilm activity. We investigated the influence of a plant polyphenolic compound resveratrol (representative of the stilbene family) on the opportunistic pathogen Trichosporon cutaneum. Besides the influence on the planktonic cells of T. cutaneum, the ability to inhibit biofilm formation and to eradicate mature biofilm was studied. We have tested resveratrol as pure compound, as well as resveratrol in complex plant extract-the commercially available dietary supplement Regrapex-R-forte, which contains the extract of Vitis vinifera grape and extract of Polygonum cuspidatum root. Regrapex-R-forte is rich in stilbenes and other biologically active substances. Light microscopy imaging, confocal microscopy, and crystal violet staining were used to quantify and visualize the biofilm. The metabolic activity of biofilm-forming cells was studied by the tetrazolium salt assay. Amphotericin B had higher activity against planktonic cells; however, resveratrol and Regrapex-R-forte showed anti-biofilm effects, both in inhibition of biofilm formation and in the eradication of mature biofilm. The minimum biofilm eradicating concentration (MBEC80) for Regrapex-R-forte was found to be 2222 mg/L (in which resveratrol concentration is 200 mg/L). These methods demonstrated that Regrapex-R-forte can be employed as an anti-biofilm agent, as it has similar effect as amphotericin B (MBEC80 = 700 mg/L), which is routinely used in clinical practice.
- MeSH
- Amphotericin B pharmacology MeSH
- Antifungal Agents pharmacology MeSH
- Biofilms drug effects growth & development MeSH
- Microbial Sensitivity Tests MeSH
- Resveratrol pharmacology MeSH
- Fallopia japonica chemistry MeSH
- Plant Extracts pharmacology MeSH
- Trichosporon drug effects growth & development metabolism MeSH
- Vitis chemistry MeSH
- Publication type
- Journal Article MeSH
- MeSH
- Fallopia multiflora * chemistry MeSH
- Phytotherapy MeSH
- Plants, Medicinal MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- MeSH
- Phytotherapy MeSH
- Plants, Medicinal MeSH
- Humans MeSH
- Fallopia japonica * chemistry MeSH
- Check Tag
- Humans MeSH
Knotweeds (Reynoutria Houtt.) are plants native to the Far East. Japanese knotweed was introduced from Japan to the unsuspecting West by the horticultural activities of Philippe von Siebold via his nursery at Leiden in the 1840s. By 1854, the plant had arrived at the Royal Botanic Gardens in Edinburgh. The plants were then sold by a large number of commercial nursery gardens around the country. Further vegetative spread followed naturally along watercourses. The knotweed is currently extremely persistant invasive plant. There is also an important source of many bioactive substances which could be used in biomedicine. The article discusses biomedically relevant constituents and its pharmacological and toxicological properties.
- Keywords
- křídlatka japonská, polydatin,
- MeSH
- Anti-Inflammatory Agents, Non-Steroidal MeSH
- Anticarcinogenic Agents MeSH
- Antimutagenic Agents MeSH
- Antioxidants MeSH
- Antineoplastic Agents, Phytogenic MeSH
- Phytotherapy MeSH
- Glucosides pharmacology chemistry therapeutic use MeSH
- Platelet Aggregation Inhibitors MeSH
- Enzyme Inhibitors MeSH
- Plant Roots MeSH
- Plants, Medicinal MeSH
- Drugs, Chinese Herbal MeSH
- Humans MeSH
- Plant Leaves MeSH
- Polygonaceae MeSH
- Resveratrol MeSH
- Fallopia japonica * MeSH
- Plant Extracts MeSH
- Stilbenes pharmacology chemistry therapeutic use MeSH
- Medicine, Chinese Traditional * MeSH
- Introduced Species MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- MeSH
- Antioxidants chemistry isolation & purification MeSH
- Chemistry Techniques, Analytical methods MeSH
- Financing, Organized MeSH
- Regression Analysis MeSH
- Fallopia japonica chemistry MeSH
- Models, Statistical MeSH
- Stilbenes chemistry isolation & purification MeSH
- Chromatography, High Pressure Liquid methods utilization MeSH