This publication presents the preparation of a certi-fied methodology for in vitrotransdermal absorption test-ing of chemicals and highlights the various pitfalls in their implementation. Vertical diffusion cells (Franz cells) were used to test the dermal absorption of caffeine, benzoic acid, and testosterone across a penetration membrane (porcine ear skin), while the receptor fluid samples were evaluated by HPLC. The designed methodology was certi-fied in 2022 in the Good Laboratory Practice system and will be used at the VUOS Rybitví and at the Medical Faculty of the Charles University in Hradec Králové to assess the dermal absorption of various substances in the environment and occupational surroundings.
The aim of this study is to estimate dietary intake of benzoic acid and its salts through food additives in adult population of South East Serbia. Information on dietary intake among 620 adults (aged 18–65) was collected using a food frequency questionnaire, and 748 food samples were analyzed. The mean estimated intake of benzoic acid – 0.32 mg/kg of body weight (bw) per day was below acceptable daily intake (ADI). Dietary exposure to benzoic acid (0.36 mg/kg of bw/day; 7.2% ADI) (consumer only), also did not exceed ADI. The main contributors of benzoic acid to dietary intake were non alcoholic beverages (43.1%), ketchup and tomato products (36.1%), and domestic pickled vegetables (19.4%). The results of this study indicate that dietary exposure to benzoic acid and its salts through food preservatives does not represent a public health risk for the adult population of South East Serbia.
- MeSH
- dieta MeSH
- dospělí MeSH
- kyselina benzoová analýza aplikace a dávkování MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- potravinářské přísady MeSH
- senioři MeSH
- vystavení vlivu životního prostředí MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Srbsko MeSH
