Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were identified to the species level using the biological method with evaluation by Farmer matrix. MALDI-TOF MS exhibited better identification capability, the data being more compact; the method was unambiguously successful in typing 145 (95 %) strains. Comparison of the results of identification by the two methods revealed differences (for 12 samples) in identified species which, considering all biochemical and/or MS characteristics, could be attributed to the natural variability of strains and close relation of the misidentified species (all of them belonged to the Citrobacter freundii complex). Taking into account all the above data, both methods can be considered reliable; however, the MALDI-TOF MS exhibits higher accuracy, efficiency, and rapidity.
- MeSH
- bakteriologické techniky MeSH
- Citrobacter klasifikace metabolismus MeSH
- enterobakteriální infekce diagnóza mikrobiologie MeSH
- lidé MeSH
- nemoci střev diagnóza mikrobiologie MeSH
- reagenční diagnostické soupravy MeSH
- Salmonella klasifikace metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
Fourteen of 21 strains isolated from stools, urine specimens and the hospital environment were identified as Kluyvera. All of these 14 strains corresponded with the literary description of the genus Kluyvera and were identical with two reference strains except that one of them failed to utilize sodium acetate within 7 days. One strain (No. 23441) produced massive growth on Jordan's tartrate, which some Kluyvera do. Important in differentiating indole- and Simmons' citrate-negative Kluyvera strains from Escherichia vulneris (two other of our 21 strains) is negative ornithin decarboxylase and negative Christensen citrate in the latter. Three strains were identified as Citrobacter, where especially indole-positive and urease-negative strains may be reminiscent of Kluyvera. An aberrant strain, No. 25115, which alone failed to grow at 42 degrees C and by some characters differed from Kluyvera, E. vulneris and Enteric Group 10, was identified as E. coli.
- MeSH
- acetáty metabolismus MeSH
- bakteriurie mikrobiologie MeSH
- citráty metabolismus MeSH
- Citrobacter klasifikace metabolismus MeSH
- Enterobacteriaceae klasifikace enzymologie růst a vývoj izolace a purifikace metabolismus MeSH
- Escherichia klasifikace metabolismus MeSH
- feces mikrobiologie MeSH
- indoly metabolismus MeSH
- kyselina octová MeSH
- lidé MeSH
- ornithindekarboxylasa analýza MeSH
- tartaráty metabolismus MeSH
- ureasa analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
