Seminal plasma (SP) provides essential nutrients, transport, and protection to the spermatozoa during their journey through the male and female reproductive tracts. Extracellular vesicles (EVs) are one of the main components of the SP with several biomolecular cargoes, including miRNAs, that can influence spermatozoa functions and interact with the cells of the female reproductive tract. This study aimed to isolate, characterize, and identify the miRNA expression profiles in the SP-EVs isolated from fertile (F) and subfertile (S) rabbit bucks that could serve as fertility biomarkers. In this study, the methods to isolate and identify EVs including exosomes, from SP of 3 F and S bucks have been developed. Ultracentrifugation and size exclusion chromatography analysis were using to isolate EVs from SP of F and S males that were qualitative and quantitively characterised using transmission electron microscopy, nanoparticle tracking analysis and western blotting. In addition, total RNA, including miRNA, was isolated, sequenced and identified from SP-EVs samples. Different SP-EVs concentrations (8.53 × 1011 ± 1.04 × 1011 and 1.84 × 1012 ± 1.75 × 1011 particles/mL of SP; P = 0.008), with a similar average size (143.9 ± 11.9 and 115.5 ± 2.4 nm; P = 0.7422) in F and S males, respectively was observed. Particle size was not significantly correlated with any kinetic parameter. The concentration of SP-EVs was positively correlated with the percentage of abnormal forms (r = 0.94; P < 0.05) and with the percentage of immotile spermatozoa (r = 0.88; P < 0.05). Small-RNA-seq analysis identified a total of 267 and 244 expressed miRNAs in the F and S groups, respectively. Two miRNAs (let-7b-5p and let-7a-5p) were the top most abundant miRNAs in both groups. Differential expression analysis revealed that 9 miRNAs including miR-190b-5p, miR-193b-5p, let-7b-3p, and miR-378-3p, and another 9 miRNAs including miR-7a-5p, miR-33a-5p, miR-449a-5p, and miR-146a-5p were significantly up- and downregulated in the F compared to the S group, respectively. The SP from F and S rabbit males contains EVs with different miRNA cargo correlated with spermatogenesis, homeostasis, and infertility, which could be used as biomarkers for male fertility and potential therapies for assisted reproductive technologies.
Models of sexually transmitted infections have become a fixture of mathematical epidemiology. A common attribute of all these models is treating reproduction and mating, and hence pathogen transmission, as uncoupled events. This is fine for humans, for example, where only a tiny fraction of sexual intercourses ends up with having a baby. But it can be a deficiency for animals in which mating and giving birth are tightly coupled, and mating thus mediates both reproduction and pathogen transmission. Here, we model dynamics of sterilizing, sexually transmitted infections in such animals, assuming structural consistency between the processes of reproduction and pathogen transmission. We show that highly sterilizing, sexually transmitted pathogens trigger bistability in the host population. In particular, the host population can end up in two extreme alternative states, disease-free persistence and pathogen-driven extinction, depending on its initial state. Given that sterilizing, sexually transmitted infections that affect animals are abundant, our results might implicate an effective pest control tactic that consists of releasing the corresponding pathogens, possibly after genetically enhancing their sterilization power.
- MeSH
- biologické modely * MeSH
- infertilita mikrobiologie veterinární MeSH
- populační dynamika MeSH
- sexuálně přenosné nemoci mikrobiologie prevence a kontrola veterinární MeSH
- sexuální chování zvířat * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- MeSH
- infertilita terapie veterinární MeSH
- skot MeSH
- Check Tag
- skot MeSH
Hlavním činitelem z vnějších faktorů, které ovlivňují podstatnou měrou plodnost, je ošetřování a životospráva hovězího dobytka. Krmení je ve svých důsledcích rozhodujícím činitelem prostředí na plodnost krav; bývá však často podceňován. Boj proti neplodnosti nesmí být problémem jen léčení chorob, ale musí probíhat na široké chovatelské frontě, začínajíc odchovem telat přes ošetřování a krmení mladého dobytka, připouštěním a průběhem porodu, stejně tak jeho stupňováním mléčné produkce
V článku probrány zásadní chyby, kterých se inseminační technici při osemeňování dopouštějí: a) znečištění kapiláry dotykem na stydké pysky, b) nedodržování vagino-rektálního způsobu osemeňování, c) osemeňování do dělohy|, d) rychlé osemeňování - šokování semene, e) jednorázová reinseminace
- MeSH
- infertilita prevence a kontrola veterinární MeSH
- inseminace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Studie vlivu slintavky v terénu na plodnost krav. Jedná se hlavně o poškození vaječníku či jiných žláz vnitřně sekretorických slintavkovým virusem
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- dospělí MeSH
- infertilita veterinární MeSH
- slintavka a kulhavka MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- zvířata MeSH
