Elevated summer temperature is reported to be the leading cause of stress in dairy and beef cows, which negatively affects various reproductive functions. Follicular cells respond to heat stress (HS) by activating the expression of heat shock family proteins (HSPs) and other antioxidants. HS is reported to negatively affect the bi-directional communication between the follicular cells and the oocyte, which is partly mediated by follicular fluid extracellular vesicles (EVs) released from surrounding cells. As carriers of bioactive molecules (DNA, RNA, protein, and lipids), the involvement of EVs in mediating the stress response in follicular cells is not fully understood. Here we used an in vitro model to decipher the cellular and EV-coupled miRNAs of bovine granulosa cells in response to HS. Moreover, the protective role of stress-related EVs against subsequent HS was assessed. For this, bovine granulosa cells from smaller follicles were cultured in vitro and after sub-confluency, cells were either kept at 37 °C or subjected to HS (42 °C). Results showed that granulosa cells exposed to HS increased the accumulation of ROS, total oxidized protein, apoptosis, and the expression of HSPs and antioxidants, while the viability of cells was reduced. Moreover, 14 and 6 miRNAs were differentially expressed in heat-stressed granulosa cells and the corresponding EVs, respectively. Supplementation of stress-related EVs in cultured granulosa cells has induced adaptive response to subsequent HS. However, this potential was not pronounced when the cells were kept under 37 °C. Taking together, EVs generated from granulosa cells exposed to HS has the potential to shuttle bioactive molecules to recipient cells and make them robust to subsequent HS.
- MeSH
- apoptóza MeSH
- extracelulární vezikuly genetika metabolismus patologie MeSH
- folikulární buňky metabolismus patologie MeSH
- nemoci skotu epidemiologie genetika prevence a kontrola MeSH
- ovariální folikul metabolismus patologie MeSH
- poruchy vyvolané tepelným stresem genetika patofyziologie veterinární MeSH
- reakce na tepelný šok * MeSH
- regulace genové exprese MeSH
- skot MeSH
- stanovení celkové genové exprese MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14-15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC).
- MeSH
- infestace klíšťaty epidemiologie imunologie prevence a kontrola veterinární MeSH
- klíšťata imunologie MeSH
- nemoci skotu epidemiologie prevence a kontrola MeSH
- skot MeSH
- vakcíny imunologie izolace a purifikace MeSH
- výzkumní pracovníci MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- kongresy MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Maroko MeSH
Ixodes ricinus is one the most abundant tick species in Europe and these ticks transmit pathogens causing human and animal diseases. The cattle ticks, Rhipicephalus (Boophilus) spp., affect cattle production in tropical and subtropical regions of the world. Development of vaccines directed against tick proteins may reduce tick infestations and the transmission of tick-borne pathogens. However, a limiting step in tick vaccine development has been the identification of tick protective antigens. Herein, the tick iron metabolism pathway was targeted in an effort to identify new tick protective antigens. Recombinant I. ricinus (IrFER2) and Rhipicephalus microplus (RmFER2) ferritin 2 proteins were expressed in Escherichia coli and used to immunize rabbits and cattle, respectively. Vaccination with IrFER2 reduced I. ricinus tick numbers, weight and fertility in rabbits with an overall vaccine efficacy (E) of 98%. Control of cattle tick, R. microplus and Rhipicephalus annulatus infestations was obtained in vaccinated cattle with overall E of 64% and 72%, respectively. Notably, the efficacy of the RmFER2 vaccine was similar to that obtained with Bm86 against R. microplus. These collective results demonstrated the feasibility of using ferritin 2 to develop vaccines for the control of tick infestations.
- MeSH
- Escherichia coli genetika MeSH
- ferritin antagonisté a inhibitory imunologie izolace a purifikace MeSH
- hmyzí proteiny antagonisté a inhibitory imunologie izolace a purifikace MeSH
- infestace klíšťaty prevence a kontrola veterinární MeSH
- klíště imunologie MeSH
- králíci parazitologie MeSH
- nemoci skotu parazitologie prevence a kontrola MeSH
- Rhipicephalus imunologie MeSH
- skot MeSH
- syntetické vakcíny imunologie MeSH
- zvířata MeSH
- Check Tag
- králíci parazitologie MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- chaperon hsp60 genetika MeSH
- DNA vakcíny imunologie MeSH
- finanční podpora výzkumu jako téma MeSH
- fungální vakcíny MeSH
- morčata MeSH
- nemoci skotu prevence a kontrola MeSH
- skot imunologie MeSH
- tinea prevence a kontrola veterinární MeSH
- Trichophyton imunologie MeSH
- zvířata MeSH
- Check Tag
- morčata MeSH
- skot imunologie MeSH
- zvířata MeSH
- MeSH
- fosfáty aplikace a dávkování MeSH
- hořčík škodlivé účinky MeSH
- látky znečišťující životní prostředí škodlivé účinky MeSH
- nemoci skotu chemicky indukované prevence a kontrola MeSH
- průjem prevence a kontrola veterinární MeSH
- skot MeSH
- vápník aplikace a dávkování MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- zvířata MeSH
- MeSH
- cysticercus imunologie MeSH
- cysticerkóza prevence a kontrola veterinární MeSH
- ELISA MeSH
- feces parazitologie MeSH
- nemoci skotu prevence a kontrola MeSH
- odpadní vody MeSH
- protilátky analýza MeSH
- průmyslová hnojiva MeSH
- siláž MeSH
- skot MeSH
- zoonózy MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
