V závěru roku 2019 proběhla v Praze konference „Rozhodněte se pro zdravé střevo“, věnovaná tématům mikrobioty (přirozeného osídlení střeva mikroorganismy) a prospěšné roli, kterou může hrát užívání lipozomálního bovinního kolostra a vitaminu C pro zdravotní stav člověka. Akci uspořádala společnost Edukafarm 9. listopadu 2019 v konferenčním sále hotelu Jalta. Přinášíme referát o hlavních informacích, které na konferenci zazněly.
BACKGROUND: Optimization of the immunoglobulin (Ig) yield in bovine milk used as therapeutic immune milk or whey for the prevention of Clostridium difficile-associated diarrhea in humans is of great importance to improve the economic efficiency of production. Individual dairy cows have diverse immune responses upon vaccination, resulting in a variable Ig yield in blood and milk. Therefore, it is advisable to pre-select cows with the best ability to produce and secrete high yields of specific Igs. RESULTS: The gene expression profile of pbMEC (primary bovine mammary epithelial cells), challenged with the gram-positive, non-mastitis, pathogen Clostridium difficile showed distinct and significant differences in the gene expression of effector molecules of the innate immune system. A number of genes were identified that could possibly serve as molecular biomarkers to differentiate high responder cows from low responder cows. These identified genes play key roles in the promotion of innate immunity. CONCLUSION: Using a gene expression profiling approach, we showed that upon others, especially the gene expression of the pro-inflammatory cytokines was altered between the high and low responder cows. Those genes are indicated as potential molecular biomarkers in the pre-selection of cows that are able to secrete high immunoglobulin yields in milk.
- MeSH
- biologické markery * MeSH
- Clostridioides difficile imunologie MeSH
- cytokiny metabolismus MeSH
- epitelové buňky imunologie MeSH
- imunoglobuliny genetika metabolismus MeSH
- mléčné žlázy zvířat imunologie MeSH
- mléko imunologie MeSH
- přirozená imunita MeSH
- skot genetika imunologie MeSH
- stanovení celkové genové exprese veterinární MeSH
- zvířata MeSH
- Check Tag
- skot genetika imunologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- apoptóza genetika MeSH
- elektronová mikroskopie MeSH
- finanční podpora výzkumu jako téma MeSH
- mléčné žlázy zvířat cytologie MeSH
- neutrofily MeSH
- průtoková cytometrie MeSH
- skot imunologie krev MeSH
- zvířata MeSH
- Check Tag
- skot imunologie krev MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- chaperon hsp60 genetika MeSH
- DNA vakcíny imunologie MeSH
- finanční podpora výzkumu jako téma MeSH
- fungální vakcíny MeSH
- morčata MeSH
- nemoci skotu prevence a kontrola MeSH
- skot imunologie MeSH
- tinea prevence a kontrola veterinární MeSH
- Trichophyton imunologie MeSH
- zvířata MeSH
- Check Tag
- morčata MeSH
- skot imunologie MeSH
- zvířata MeSH
Sera of 259 persons, 801 sheep, 54 goats and 60 cows from several regions of Czechoslovakia were examined by tube-neutralization test (TNT) and haemagglutination-inhibition test (HIT) for antibodies against Bhanja virus. The results of both assays correlated well, but TNT was a more sensitive test than HIT. Antibodies neutralizing Bhanja virus were detected in 2.7% of persons, 5.1% of sheep, 18.5% of goats and 16.7% of cattle, most frequently in the district Roznava (Eastern Slovakia), where 38.5% of goats, 26.7% of sheep, 16.7% of cattle and 9.5% humans (predominantly goat or sheep breeders) were positive. A recent circulation of the virus in natural foci of that area is revealed by the detection of antibodies in several calves and lambs, which grazed in one year (1982) only. Roznava district is also unique (among the areas examined) in the presence of the tick Haemaphysalis punctata, principal vector of Bhanja virus in Europe.
- MeSH
- arachnida jako vektory MeSH
- Bunyaviridae imunologie MeSH
- druhová specificita MeSH
- klíšťata MeSH
- kozy imunologie MeSH
- lidé MeSH
- neutralizační testy MeSH
- ovce imunologie MeSH
- protilátky virové analýza MeSH
- skot imunologie MeSH
- testy inhibice hemaglutinace MeSH
- zdroje nemoci MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot imunologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
