- MeSH
- Bacteria patogenita MeSH
- bakteriální proteiny chemie MeSH
- disulfidy chemie metabolismus MeSH
- Escherichia coli enzymologie patogenita MeSH
- financování organizované MeSH
- Francisella tularensis enzymologie genetika patogenita MeSH
- isomerasy chemie MeSH
- membránové proteiny chemie MeSH
- molekulární modely MeSH
- molekulární sekvence - údaje MeSH
- Neisseria meningitidis enzymologie genetika patogenita MeSH
- oxidace-redukce MeSH
- proteindisulfidisomerasy chemie MeSH
- proteiny z Escherichia coli chemie MeSH
- proteiny chemie MeSH
- sbalování proteinů MeSH
- stabilita proteinů MeSH
- virulence MeSH
- Yersinia pestis enzymologie genetika patogenita MeSH
- Publikační typ
- přehledy MeSH
We determined the role of Yersinia pestis virulence markers in an animal model of pneumonic plague. Eleven strains of Y. pestis were characterized using PCR assays to detect the presence of known virulence genes both encoded by the three plasmids as well as chromosomal markers. The virulence of all Y. pestis strains was compared in a mouse model for pneumonic plague. The presence of all known virulence genes correlated completely with virulence in the Balb/c mouse model. Strains which lacked HmsF initially exhibited visible signs of disease whereas all other strains (except wild-type strains) did not exhibit any disease signs. Forty-eight hours post-infection, mice which had received HmsF(-) strains regained body mass and were able to control infection; those infected with strains possessing a full complement of virulence genes suffered from fatal disease. The bacterial loads observed in the lung and other tissues reflected the observed clinical signs as did the cytokine changes measured in these animals. We can conclude that all known virulence genes are required for the establishment of pneumonic plague in mammalian animal models, the role of HmsF being of particular importance in disease progression.
- MeSH
- analýza přežití MeSH
- bakteriální geny MeSH
- bakteriální nálož MeSH
- cytokiny sekrece MeSH
- DNA bakterií genetika MeSH
- faktory virulence genetika metabolismus MeSH
- modely nemocí na zvířatech MeSH
- mor mikrobiologie mortalita patologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nemoci hlodavců mikrobiologie mortalita patologie MeSH
- plazmidy analýza MeSH
- plíce mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- proteiny vnější bakteriální membrány genetika metabolismus MeSH
- tělesná hmotnost MeSH
- virulence MeSH
- Yersinia pestis genetika patogenita MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH