The aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 mecA-positive and 14 mecA-negative) of animal origin previously identified as Staphylococcus intermedius by phenotypic tests and species-specific PCR amplification of the 16S rRNA gene. For this purpose, we used a multiplex PCR for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by PCR. Both methods allow discrimination of Staphylococcus pseudintermedius from the other closely related members of the S. intermedius group and other coagulase-positive staphylococci isolated from animals. Genetic diversity of S. pseudintermedius strains was analyzed by staphylococcal protein A-encoding gene (spa) typing. Multiplex PCR method was used to identify staphylococcal cassette chromosome mec (SCCmec) type in mecA-positive strains. All isolates previously identified as S. intermedius were shown to belong to S. pseudintermedius. According to PCR-based SCCmec typing, SCCmecIII was the most prevalent type (n = 23), and solely seven isolates were designated as non-typeable. Furthermore, the assessment of spa-typing results revealed that the majority of all strains (n = 27) harbored spa type t02, and 17 strains were classified as non-typeable.
- MeSH
- bakteriální léková rezistence MeSH
- bakteriální proteiny analýza genetika MeSH
- Gerbillinae MeSH
- kočky MeSH
- králíci MeSH
- mikrokoková nukleasa analýza genetika MeSH
- multiplexová polymerázová řetězová reakce MeSH
- nemoci zvířat epidemiologie mikrobiologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- psi MeSH
- RNA ribozomální 16S analýza MeSH
- skot MeSH
- stafylokokové infekce epidemiologie mikrobiologie veterinární MeSH
- stafylokokový protein A analýza genetika MeSH
- Staphylococcus intermedius klasifikace genetika izolace a purifikace patogenita MeSH
- techniky typizace bakterií MeSH
- tělesné tekutiny mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- králíci MeSH
- psi MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Polsko MeSH
A rapid test for the detection of staphylococcal thermostable nuclease (TNase) is described. The procedure consists of heat inactivation of solid cultures of staphylococci and microslide agar diffusion in toluidine blue agar containing deoxyribonucleic acid. Using this method the results are obtained about 1 d sooner than with the conventional method.
