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MeSH: tukové buňky-transplantace

2 záznamů v Medvik Filtry

Článek

Laminin-Alginate Beads as Preadipocyte Carriers to Enhance Adipogenesis In Vitro and In Vivo

Hsueh, Yu-Sheng
Autor Hsueh, Yu-Sheng Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan . 2 Taiwan Food and Drug Administration, Ministry of Health and Welfare , Taipei, Taiwan
Chen, Yo-Shen
Autor Chen, Yo-Shen Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan . 3 Department of Plastic Surgery, Far Eastern Memorial Hospital , New Taipei City, Taiwan
Tai, Hao-Chih
Autor Tai, Hao-Chih Department of Plastic Surgery, National Taiwan University Hospital , Taipei, Taiwan
Mestak, Ondrej
Autor Mestak, Ondrej Department of Plastic Surgery, First Faculty of Medicine, Charles University in Prague , Bulovka Hospital, Prague, Czech Republic
Chao, Sung-Chuan
Autor Chao, Sung-Chuan Department of Surgery, National Taiwan University Hospital , Taipei, Taiwan
Chen, Yen-Yu
Autor Chen, Yen-Yu Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan
Shih, Ying
Autor Shih, Ying Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan
Lin, Jung-Feng
Autor Lin, Jung-Feng Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan
Shieh, Ming-Jium
Autor Shieh, Ming-Jium Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan
Lin, Feng-Huei
Autor Lin, Feng-Huei Institute of Biomedical Engineering, National Taiwan University , Taipei, Taiwan . 7 Division of Medical Engineering, National Health Research Institute , Maoli, Taiwan

Tissue engineering. Part A. 2017 ; 23 (5-6) : 185-194. [pub] 20161213

Tissue Eng Part A
ISSN 1937-335X
Medvik
Zdroj

The use of autologous fat grafting in breast reconstruction still requires optimization. Fat survival and calcification are the main issues that affect the outcomes of the procedure. In this study, a cell-based therapy utilizing laminin-alginate beads (LABs) as carriers was proposed to promote cell survival and adipogenesis by providing short-term physical support and facilitate nutrient diffusion of the implants. Laminin-modified alginate beads were fabricated by immobilizing laminin onto ring-opened alginate, used to encapsulate 3T3-L1 preadipocytes, and evaluated in vitro and in vivo. LABs as preadipocyte carriers showed better biocompatibility and stability than unmodified alginate beads. Preadipocytes in LABs had higher survival rate and enhanced adipogenesis than those in unmodified alginate beads. In vivo studies showed that LABs gradually degraded and the sites were replaced by newly formed fat tissues, and new blood vessels were also observed. 7T-MRI study mimicking clinical fat grafting showed that LABs carrying adipose stem cells improved the results of conventional fat grafts. Therefore, we believe that LABs represent promising cell carriers and can be potentially used for the reconstruction of breasts or other soft tissues in the future.