BACKGROUND: The aim of our study was to assess the impact of different thawing protocols on morphological changes arising in cryopreserved human saphenous vein grafts. METHODS: The study was performed in 12 saphenous vein grafts harvested in brain death donors. Storage in the vapor phase of liquid nitrogen for 3 or 5 years followed. Two thawing protocols were tested: slow thawing in a refrigerator at temperature +4°C for 2 hr and rapid thawing-in a water bath at +37°C. Grafts were processed for scanning electron microscopy. Comparisons of continuous parameters under study between experimental groups were performed using the t-test (age, cold ischemia time, exposure to cryoprotectant, time of storage, total thawing time, mean thawing rate, morphology scoring of thawed HSVG) and the median test (HSVG length). Categorical parameters (sex and blood group) were formally tested using the chi-square test. RESULTS: All samples were evaluated according to morphological changes and scored in terms of morphologically intact endothelium, confluent endothelium with structural inhomogeneity, disruption of the intercellular contacts, separation of the endothelial cells, complete loss of the endothelium, and damage of the subendothelial layers. There is no statistically significant difference between the sample sets at the significance level of 0.05. There was no association with donors' age, sex, and time of storage. CONCLUSIONS: Human cryopreserved saphenous vein grafts in our experimental work showed no difference in terms of structural deterioration of the endothelial surface and basal membrane depending on different thawing protocols used.
- MeSH
- časové faktory MeSH
- dospělí MeSH
- endoteliální buňky účinky léků transplantace ultrastruktura MeSH
- kryoprezervace * MeSH
- kryoprotektivní látky farmakologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- odběr tkání a orgánů MeSH
- přežití tkáně MeSH
- vena saphena účinky léků transplantace ultrastruktura MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- MeSH
- dolní končetina ultrasonografie MeSH
- duplexní dopplerovská ultrasonografie metody využití MeSH
- lidé MeSH
- směrnice pro lékařskou praxi jako téma MeSH
- tromboflebitida ultrasonografie MeSH
- varixy ultrasonografie MeSH
- vena femoralis ultrasonografie MeSH
- vena poplitea ultrastruktura MeSH
- vena saphena ultrastruktura MeSH
- žilní insuficience ultrasonografie MeSH
- Check Tag
- lidé MeSH
- MeSH
- lidé MeSH
- mikroskopie metody MeSH
- varixy patologie MeSH
- vena saphena patologie ultrastruktura MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- kongresy MeSH
