2-chloroethyl ethyl sulfide (CEES) is a vesicant agent, commonly referred to half mustard due to its ability to form monofunctional adducts with DNA. In this study, we evaluated the chemoprotective potential of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate (MESNA) against CEES-induced geno- and cytotoxicity in human lung cell line A-549. MESNA, L-glutathione (GSH), thiourea, sodium thiosulfate, hexamethylenetetramine, 4-acetamidophenol, asoxime dichloride (HI-6), N-acetyl-L-cysteine (NAC), sodium pyruvate, myo-inositol, 3-aminobenzamide (3-AB), nicotinamide, and Nω-nitro-L-arginine methyl ester hydrochloride and combinations of these compounds with MESNA were applied 30 min before CEES. DNA alkylation was measured using modified comet assay 1 and 24 h after the exposure. Cell viability was determined using MTT assay at 24 and 72 h. The mono-therapeutical approach identified MESNA and GSH to provide significant chemoprotection. NAC and 3-AB supported DNA damage repair, while cell viability remained unaffected. Mixtures of GSH or NAC with MESNA showed protective synergism against DNA damage. Other compounds or their combinations with MESNA failed due to the potentiation of CEES-induced cytotoxicity. The chemoprotection against CEES remains limited; however, the combination of substances can provide protective synergy and may represent a promising strategy in the treatment of accidental exposure to monoalkylating agents.
- MeSH
- antidota MeSH
- chemické bojové látky toxicita MeSH
- chemoprofylaxe MeSH
- kometový test MeSH
- kultivované buňky účinky léků MeSH
- mesna analogy a deriváty terapeutické užití MeSH
- poškození DNA účinky léků MeSH
- testy genotoxicity metody MeSH
- yperit * analogy a deriváty toxicita MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- adjuvantní chemoterapie metody MeSH
- antagonisté kyseliny listové dějiny farmakologie terapeutické užití MeSH
- cílená molekulární terapie metody MeSH
- cisplatina dějiny farmakologie škodlivé účinky terapeutické užití MeSH
- kombinovaná terapie metody trendy MeSH
- lidé MeSH
- methotrexát dějiny farmakologie terapeutické užití MeSH
- neoadjuvantní terapie metody MeSH
- protinádorové látky * dějiny farmakologie terapeutické užití MeSH
- taxoidy dějiny farmakologie terapeutické užití MeSH
- yperit analogy a deriváty dějiny farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
