Q11718096
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The aim of our study was to examine the direct influence of plant polyphenol resveratrol and oil-related environmental contaminant benzene on ovarian hormone release, as well as the ability of resveratrol to prevent the effect of benzene. Porcine ovarian granulosa cells were cultured with and without resveratrol (0, 1,10 or 100 ug/ml) alone or in combination with 0.1% benzene. The release of progesterone, oxytocin and prostaglandin F was measured by enzyme immunoassay (EIA). Benzene promoted the release of progesterone, oxytocin and prostaglandin F. Resveratrol, when given alone, stimulated both progesterone and prostaglandin F, but not the oxytocin output. Moreover, resveratrol prevented and even inverted the stimulatory action of benzene on all analysed hormones. These observations demonstrate the direct influence of both benzene and resveratrol on porcine ovarian hormone release, as well as the ability of resveratrol to prevent the benzene action on the ovary.
- MeSH
- benzen farmakologie MeSH
- folikulární buňky cytologie účinky léků metabolismus MeSH
- kultivované buňky MeSH
- oxytocin metabolismus MeSH
- prasata MeSH
- progesteron metabolismus MeSH
- prostaglandiny F metabolismus MeSH
- resveratrol farmakologie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- apoptóza účinky záření MeSH
- folikulární buňky fyziologie účinky léků MeSH
- guanosinmonofosfát cyklický analogy a deriváty fyziologie MeSH
- inhibitory enzymů MeSH
- progesteron sekrece MeSH
- proteinkinasy závislé na cyklickém GMP antagonisté a inhibitory fyziologie MeSH
- skot MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
Journal of physiology and pharmacology. 1, ISSN 0867-5910 Supplement Vol. 47
171 s. ; 24 cm
- MeSH
- fertilizace in vitro MeSH
- těhotenství u zvířat MeSH
- Publikační typ
- kongresy MeSH
- Konspekt
- Lékařské vědy. Lékařství
- NLK Obory
- fyziologie
The present study examined the effect of medicinal plants - ginkgo, tribulus (puncture vine), and yucca - on ovarian functions and their response to the toxic influence of toluene. Therefore, we analyzed the effect of toluene with and without these plant extracts on cultured human ovarian granulosa cells. Cell viability and the release of progesterone, insulin-like growth factor I (IGF I), oxytocin, and prostaglandin F (PGF) were analyzed using the trypan blue test, enzyme immunoassay, and enzyme-linked immunosorbent assay, respectively. The ginkgo, tribulus and yucca were able to suppress ovarian cell viability and alter the release of hormones. Toluene suppressed cell viability and the release of PGF, but not of progesterone, IGF-I, or oxytocin. The negative effect of toluene on cell viability was prevented and even reversed by ginkgo and yucca, whereas its effect on PGF was prevented or inverted by all tested plant extracts. These findings (1) demonstrated the direct toxic effect of toluene on ovarian cells, (2) showed the direct effect of some medicinal plants on ovarian cell functions, and (3) demonstrated the ability of these plants to inhibit the effects of toluene and to act as natural protectors against the suppressive effect of toluene on female reproduction.
- MeSH
- léčivé rostliny * MeSH
- lidé MeSH
- oxytocin MeSH
- progesteron MeSH
- rostlinné extrakty farmakologie MeSH
- viabilita buněk MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- AMP cyklický metabolismus MeSH
- buněčné dělení účinky léků MeSH
- insulinu podobný růstový faktor I farmakologie MeSH
- krevní proteiny farmakologie MeSH
- ovariální folikul metabolismus účinky léků MeSH
- oxytocin farmakologie MeSH
- protilátky farmakologie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
