The tendency to form a beta-turn in alpha-gliadin was estimated using the B-cell determinant prediction program based on the Chou and Fasman probability of beta-turn formation. Six sequences possessing a high probability of beta-turn formation were found. A statistically high agreement was found between these six sequences and three areas in alpha-gliadin with the occurrence of Pro-Ser-Gln-Gln sequence which has recently been considered responsible for toxicity in coeliac disease. By means of solid-phase synthesis seven peptides were obtained covering the above-mentioned regions. Their toxicity was tested using the fetal chick duodenum. The results support the suggestion that peptides containing the sequences Pro-Ser-Gln-Gln and Gln-Gln-Gln-Pro may be involved in the pathogenesis of coeliac disease.

• MeSH
• celiakie etiologie   MeSH
• duodenum embryologie   účinky léků   MeSH
• gliadin * chemie   toxicita   MeSH
• kuřecí embryo MeSH
• molekulární sekvence - údaje MeSH
• zvířata * MeSH
• Check Tag
• kuřecí embryo MeSH
• zvířata * MeSH

Three dodecapeptide amides derived from the amino acid sequence of alpha-gliadin and four of their analogues were synthesized by continuous-flow solid-phase multiple peptide synthesis. Ultrasonic field conditions accelerated the coupling reaction without affecting purity. Biological tests of the synthetic fragments showed the relevance of toxicity prediction for preselection of immunogenic alpha-gliadin fragments.

• MeSH
• duodenum enzymologie   účinky léků   MeSH
• gliadin farmakologie   chemicky indukované   chemie   MeSH
• kuřecí embryo MeSH
• onkogenní proteiny virové chemie   MeSH
• peptidové fragmenty farmakologie   chemicky indukované   chemie   MeSH
• techniky in vitro MeSH
• ultrazvuk MeSH
• zvířata MeSH
• Check Tag
• kuřecí embryo MeSH
• zvířata MeSH