Belladonna Dotaz Zobrazit nápovědu
24 s. ; 21 cm
- MeSH
- Atropa belladonna MeSH
- Publikační typ
- vysokoškolské kvalifikační práce MeSH
- Konspekt
- Botanika
- NLK Obory
- botanika
- farmacie a farmakologie
- MeSH
- Atropa belladonna MeSH
- léčivé rostliny MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Klíčová slova
- Belladona 200C,
- MeSH
- Atropa belladonna * MeSH
- homeopatie MeSH
- lidé MeSH
- materia medica * aplikace a dávkování farmakologie terapeutické užití MeSH
- rostlinné extrakty * terapeutické užití MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male Sprague-Dawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action.
- MeSH
- Atropa belladonna chemie MeSH
- časové faktory MeSH
- extracelulární matrix metabolismus účinky léků MeSH
- fibroblasty metabolismus patologie účinky léků MeSH
- fibronektiny metabolismus MeSH
- galektin 1 metabolismus MeSH
- hojení ran účinky léků MeSH
- keratin-19 metabolismus MeSH
- keratinocyty metabolismus patologie účinky léků MeSH
- kolagen typ III metabolismus MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- kůže metabolismus patologie účinky léků zranění MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- penetrující rány farmakoterapie metabolismus patologie MeSH
- potkani Sprague-Dawley MeSH
- rostlinné extrakty farmakologie chemie izolace a purifikace MeSH
- rozpouštědla chemie MeSH
- vimentin metabolismus MeSH
- voda chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
