• Publication type
• Meeting Abstract MeSH

We aimed to establish an efficient RNA interference (RNAi) system in the industrially important filamentous fungus Trichoderma koningii using the DsRed protein as a reporter of the silencing process. To accomplish this, a DsRed expression cassette was transformed into T. koningii, and a recombinant strain that stably expressed DsRed was obtained. Next, a vector-directing expression of a DsRed hairpin RNA was constructed and transformed into the T. koningii recipient strain. Approximately 79 % of transformants displayed a decrease in DsRed fluorescence, and expression of DsRed in some transformants appeared to be fully suppressed. Characterization of randomly selected transformants by genomic DNA PCR analysis, real-time PCR quantification, and western blot confirmed downregulation of gene expression at different levels. The RNA silencing approach described here for T. koningii is effective, and the DsRed reporter gene provides a convenient tool for identification of silenced fungal transformants by their DsRed fluorescence compared to the control strain. The results of this study demonstrate the power of RNAi in T. koningii, which supports the use of this technology for strain development programs and functional genomics studies in industrial fungal strains.

• MeSH
• Fluorescence MeSH
• Gene Knockdown Techniques methods   MeSH
• Real-Time Polymerase Chain Reaction MeSH
• Luminescent Proteins analysis   genetics   MeSH
• RNA, Small Interfering genetics   MeSH
• Genetics, Microbial methods   MeSH
• Recombination, Genetic MeSH
• Genes, Reporter MeSH
• RNA Interference * MeSH
• Gene Expression Profiling MeSH
• Transformation, Genetic MeSH
• Trichoderma genetics   MeSH
• Blotting, Western MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Na stanovování cílů jakosti, jejího udržování, kontrolu a sledování je vypracována a používána řada sofistikovaných metod včetně statistických. V textu jsou popsány některé z těchto nástrojů, které patří k základní výbavě moderního managementu. Uvedené postupy jsou priblíženy na konkrétních příkladech i pro oblast transfuzní služby. Většina popsaných nástrojů má široké uplatnění v celé teorii a praxi řízení, nejen managementu jakosti. Základem kvalitního řízení jakosti je mít stanoveny cíle jakosti a politiku jakosti. Jsou uvedeny výhody týmové práce, je stručně popsán Demingův cyklus. Základním nástrojem pro systematické a nezávislé zkoumání dosažené úrovně jakosti jsou její prověrky - audity. Popsáno je sedm jednoduchých nástrojů na zlepšování jakosti: 1. vývojový diagram, 2. záznamníky, 3. histogram, 4. Ishikawův diagram, 5. Paretův graf, 6. bodový (korelační) diagram a 7. regulační diagram (statistická regulace procesu).

For assessment of objectives of quality, its maintenance, control and monitoring a number of sophisticated methods incl. statistical ones were elaborated and are used. The author describes some of these tools which belong to the basic equipment of modem management. The mentioned procedures are explained on actual examples incl. the transfusion service. The majority of the described tools has a wide application in the whole theory and practice of management, not only quality control. „The basis of high standard of quality management is to establish the quality aims and quality policy." The author mentions the advantages of team work, describes briefly Deming's cycle. The basic tool for systematic and independent investigation of the achieved quality level are audits. The author describes seven simple tools for improvement of quality: 1. developmental diagram, 2. recorders, 3. histogram, 4. Ishikawa diagram, 5. Pareťs graph, 6. point (correlation) diagram and 7. regulation diagram (statistical regulation of the process).

• MeSH
• Hematologic Tests standards   MeSH
• Total Quality Management MeSH
• Blood Banks economics   standards   organization & administration   MeSH
• Blood Transfusion economics   standards   MeSH
• Reference Standards MeSH
• Quality Control MeSH
• Publication type
• Guideline MeSH

Androgen receptor (AR) expression in prostate cancer (CaP) cells varies due to the multiple changes including epigenetic modifications such as DNA methylation and histone deacetylation. DNA methyltransferase and histone deacetylase inhibitors are promising for the treatment of CaP. The aim of our study was to analyze the 5-aza-2'-deoxycytidine (Aza‑dC) and sodium butyrate (NaB) effects on CaP cells with modified AR gene expression. The androgen-independent human prostate cancer cell lines PC3 (lacking a functional AR) and DU145 (strongly limited expression due to methylations in the AR gene) were used. PCR of bisulfite-modified DNA and RT-PCR with bisulfite-sequencing were used for AR gene analysis of DU145 and PC3 cells following their treatment with Aza-dC and/or NaB. Re-acetylated histones around the AR gene were detected by conventional PCR of immunoprecipitated DNA obtained from treated cells. In both cell lines without the AR expression, the combined treatment was followed with significant decrease of cell viability. The co-treatment of DU145 cells caused site-specific demethylation in the AR promoter region followed by gene re-expression and increased acetylation in histones H3 and H4. The co-treatment with Aza-dC and NaB was the most effective in demethylation and re-expression of the AR gene. In the AR gene promoter, the location and density of deme-thylated CpGs indicated the existence of distinct promoter hot spot that could be a target of AR gene inactivation therapy of CaP patients during androgen deprivation.

• MeSH
• 5' Untranslated Regions MeSH
• Acetylation drug effects   MeSH
• Receptors, Androgen biosynthesis   genetics   metabolism   MeSH
• Androgens genetics   metabolism   MeSH
• Azacitidine analogs & derivatives   pharmacology   MeSH
• DNA genetics   MeSH
• Histone Deacetylases genetics   metabolism   MeSH
• Histones genetics   metabolism   MeSH
• Histone Deacetylase Inhibitors pharmacology   MeSH
• Butyric Acid pharmacology   MeSH
• Humans MeSH
• Methyltransferases antagonists & inhibitors   genetics   metabolism   MeSH
• DNA Methylation drug effects   MeSH
• Cell Line, Tumor MeSH
• Prostatic Neoplasms, Castration-Resistant drug therapy   enzymology   genetics   metabolism   MeSH
• Promoter Regions, Genetic drug effects   genetics   MeSH
• Cell Survival drug effects   genetics   MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Řízení projektů je jedním z nejdůležitějších, přitom však nejobtížnějších manažerských úkolů - a v oblasti informačních technologií to platí dvojnásob. Proslulá kniha zkušené autorky se zabývá celým životním cyklem softwarového projektu a přináší neocenitelné rady, návody, postupy a znalosti z praktického vývoje, které zlevní, urychlí a zefektivní průběh a dodání vašich projektů.

• MeSH
• Organization and Administration MeSH
• Computers MeSH
• Publication type
• Handbook MeSH

• Conspectus
• Management. Řízení
• NML Fields
• management, organizace a řízení zdravotnictví

Výklad matematických a statistických metod, vycházející z potřeb manažerů řešit racionálně ekonomické, prognostické, kapacitní a jiné problémy.

• Keywords
• management,
• MeSH
• Mathematical Computing MeSH
• Organization and Administration MeSH
• Entrepreneurship MeSH
• Likelihood Functions MeSH
• Publication type
• Textbook MeSH

• Conspectus
• Kombinatorika. Teorie grafů. Matematická statistika. Operační výzkum. Matematické modelování
• NML Fields
• statistika, zdravotnická statistika
• ekonomie, ekonomika, ekonomika zdravotnictví