Franĕk, Frantisek*
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- MeSH
- celulosa MeSH
- chemická precipitace MeSH
- dinitrofenoly * MeSH
- gama-globuliny * MeSH
- imunoelektroforéza MeSH
- krev MeSH
- prasata MeSH
- protilátky * MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Five stable hybridoma lines were prepared using the myeloma cell line P3-X63-Ag.653 and spleen cells of mice hyperimmunized by pig transferrin. All hybridomas grew well in mouse peritoneal cavity and produced antibodies of the IgG1 subclass. Antibody preparations obtained from ascitic fluids tested for their capacity of antigen precipitation. No precipitation was obtained with single antibodies and with pairs of antibodies. Three out of 10 possible triads gave clear and sharp precipitation zones and rings in immunodiffusion tests performed in agar gel. All 5 antibodies were shown by quantitative enzyme-immunoassay to be specific for pig transferrin: no cross-reaction was obtained with mouse, human, horse and sheep transferrins.
- MeSH
- chemická precipitace MeSH
- druhová specificita MeSH
- koně MeSH
- lidé MeSH
- monoklonální protilátky * MeSH
- myši MeSH
- ovce MeSH
- prasata MeSH
- specificita protilátek MeSH
- transferin imunologie MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
b.v. 46 s.